1. From: Corneal Fibroblasts Respond Rapidly to Changes in Local Mechanical Stress
Invest. Ophthalmol. Vis. Sci ;45(10): doi: /iovs
Figure Legend:
Y and cytochalasin D blocked the cellular response to ECM micromanipulation (representative of 10 Y experiments and 4 cytochalasin D experiments). (A–D) Rabbit corneal fibroblast 1 day after plating inside collagen matrix. (B) Cell elongation (arrows) and ECM relaxation was observed after adding Y (black tracks, ×: position just before addition of Y-27632). (C) Subsequent pushing with a needle (note shadow of needle on right) induced little cell contraction (5.9% shortening, black tracks). (D) There was also no secondary spreading or traction after needle push, as indicated by minimal ECM displacement (black tracks, ×: starting position 1 minute after push). (E–H) Human corneal fibroblast 2 days after plating inside collagen matrix. (F) After cytochalasin D was added, elongation of the cell (arrows) and relaxation of cell-induced matrix stress were observed (black tracks). (G) Pushing on the ECM in front of the cell caused ECM compression in front of the needle, but little cell contraction (4.7% shortening, black tracks). (H) There was also no secondary spreading or traction after needle push, as indicated by minimal ECM displacements (black tracks, ×: starting position 1 minute after push).
Date of download: 10/15/2017
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