1. PINEAPPLE AND ENZYME LAB

2. Background Information: Ask an expert.
Gelatin makes Jello a semi-solid. 1845
Gelatin comes from bones and cartilage from pigs and cows
Enzyme Bromelain is in Pineapple
Vitamin C is a co-enzyme to Bromelain
Bromelain helps relieve pain in arthritis.
Bromelain breaks down mucus and helps those with arthritis and bronchus

3. Investigative Question:
How do different types of pineapple affect gelatin?
Hypothesis:
If pineapple is put into gelatin, then ________
LABEL YOUR LAB BOOK!

4. Enzymes are
Protein catalyst (starter) for chemical reactions cut (Catabolism) or add (Anabolism) substances in cells.

5. Anabolism: Catabolism in Jello
Anabolism: Starch (Carbohydrates) in the gelatin get thicker as it cools.
= 000
Catabolism: Bromelain (protein) in pineapple break the solution apart: (prevent it from getting thick.
000 + Protein Enzyme =

6. Materials: Fresh pineapple 2 pieces Canned Pineapple 2 pieces
Gelatin (Jell-O ingredient)
May redo with Apple, Orange or other thing you bring in. It must be authorized by the teacher
4 Dixie cups
Ice Tub
Ice
Stir sticks

7. Procedure Get 4 Dixie cups and label each with tape
* fresh * canned * no pineapple
2. Put 2 pieces (about the same amount) of each type of pineapple different Cups.
3. Take the Dixie cup to the gelatin and pour enough to cover the pineapple
4. Only pour gelatin into Dixie Cup # 4.

8. Variables Control Group: No pineapple, just gelation
(Check to see if there is something else making changes in the gelation)
Manipulative Variables: fresh and frozen pineapple, other
Responding Variable: What happens to the gelation
Experimental Controls: Same pineapple, same temperature, same gelation, same ___ same____

9. 5. Put the cups in a pan of ice with the ice to the
Top level of the gelatin/pineapple.
6. Let the Dixie cups sit in ice for up to 10 minutes checking the control every 2 minutes by swirling or stirring the Dixie cups.
7. Record observations on a data table.
8. When finished, pour semi-solid mixtures into waste beaker - NOT DOWN THE DRAIN!
9. Clean lab thoroughly.
10. Observe other groups work. Record findings

10. Variables Data Table Experimental (one tested to) Variable 1:
Responding (results) Variable:
Experimental Controls: (same/same)
Data Table
4 min
10 min
Fresh
Canned
Control

11. Analysis Questions: Describe the differences between Cups.
What was it like when you started and at the end?
Does the pineapple enzyme Bromelain breakdown (catabolism) or build (anabolism) proteins?
After looking at the data, what is the best explanation for the results of experiment?
How could you increase validity.
How could you increase reliability?

12. Conclusion Answer to the investigative question.
Data to support the answer to the investigative question.
Address any trends you see in the data. (What happened?)
Explain why the trend in data occurred.