1. Endometrial prostaglandin synthases, ovarian steroids, and oxytocin receptors in mares with oxytocin-induced luteal maintenance 
Maria R. Rebordão, António Galvão, Pedro Pinto-Bravo, Joana Pinheiro, Sandra Gamboa, Elisabete Silva, Luísa Mateus, Graça Ferreira-Dias 
Theriogenology 
Volume 87, Pages (January 2017)
DOI: /j.theriogenology
Copyright © 2016 Elsevier Inc. Terms and Conditions

2. Fig. 1
Corpus luteum (A) and uterus (B) 24-day postovulation in oxytocin-treated mares with prolonged luteal function. (C) Mean ± SEM plasma progesterone (P4) concentration in mares treated twice daily with saline (control; n = 4) or 60 units of oxytocin/mare (OXT; n = 6). OXTPLF, oxytocin-treated mares with prolonged luteal function (n = 4); OXTRE, oxytocin-treated mares that underwent luteolysis at the normal time (n = 2).
Theriogenology  , DOI: ( /j.theriogenology )
Copyright © 2016 Elsevier Inc. Terms and Conditions

3. Fig. 2
Relative quantification of gene transcription by real-time PCR of proteins involved in prostaglandin biosynthesis and oxytocin and ovarian steroids receptors in endometrial biopsies collected on Day 10 postovulation in control mares (n = 4) and on Day 24 in oxytocin-treated mares (n = 4). Expression of target genes was normalized against that of the housekeeping gene (RPL32). Bars represent mean ± SEM. Asterisks indicate significant differences (*P < 0.05; **P < 0.01). AU, arbitrary units.
Theriogenology  , DOI: ( /j.theriogenology )
Copyright © 2016 Elsevier Inc. Terms and Conditions

4. Fig. 3
Protein expression of PTGS2, oxytocin receptor (OXTR), and estrogen receptors (ESR1 and ESR2) in endometrial biopsies of control and oxytocin-treated mares. Endometrial biopsies were collected on Day 10 postovulation in control mares (n = 4) and on Day 24 in oxytocin-treated mares (n = 4). Histograms show densitometric analysis of PTGS2, OXTR, ESR1, and ESR2 proteins normalized against β-actin. Upper panels illustrate representative Western blots. The left lane of each blot is from control mares and the right from OXT-treated mares. Data represent mean ± SEM. Asterisk indicates significant differences (*P < 0.05). AU, arbitrary units.
Theriogenology  , DOI: ( /j.theriogenology )
Copyright © 2016 Elsevier Inc. Terms and Conditions

5. Fig. 4
Endometrial immunohistochemistry representative images of PTGS2, AKR1C3, PTGES, and PTGIS protein in Day 10 postovulation biopsies of mares treated with saline (control) and in Day 24 postovulation biopsies in oxytocin-treated mares (OXT). Positive staining is shown in brown. In negative controls, primary antibodies were replaced by the same concentration of rabbit or mouse immunoglobulin G. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
Theriogenology  , DOI: ( /j.theriogenology )
Copyright © 2016 Elsevier Inc. Terms and Conditions

6. Fig. 5
Endometrial immunohistochemistry representative images of PGR, OXTR, ESR1, and ESR2 protein in Day 10 postovulation biopsies of mares treated with saline (control) and in Day 24 postovulation biopsies in oxytocin-treated mares (OXT). Positive staining is shown in brown. In negative controls, primary antibodies were replaced by the same concentration of rabbit or mouse immunoglobulin G. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
Theriogenology  , DOI: ( /j.theriogenology )
Copyright © 2016 Elsevier Inc. Terms and Conditions