1. Lesson 1.2 Lactase Lab

2. Another Trait: Lactose Intolerance
People who are lactose intolerant cannot eat or drink dairy products
Symptoms:
Stomach cramps
Bloating
Diarrhea
Throwing up
Can take a supplement called lactase to help them digest dairy better.

3. So you decide you want to help people with lactose intolerance or other similar traits… What jobs could you get?

4. Congratulations on your new job…
Research Scientist
McNeil Nutritionals

5. Based in Fort Washington, Pennsylvania
Image sources:

6. Welcome to Philadelphia
Image sources:

7. Welcome to Johnson & Johnson
McNeil Nutritionals
-Splenda
-Benecol
-Lactaid
Image sources:

8. How much you get paid.. $60,000/year
Benefits package includes health, dental, vision, and life insurance; 401(k) account; and annual, sick and holiday leave.
Image source:

9. Your first project Product testing and analysis of Lactaid
People are finding that if they dissolve the Lactaid in their coffee or tea it doesn’t work
Your job: confirm this common complaint in the lab and figure out why it happens.
Image source:

10. Stuff you remember from your high school biology class…
Lactose is the sugar in milk.
Lactase is a type of protein called an enzyme that digests it.
No Lactase = Lactose Intolerance
Glucose
Galactose
Lactose

11. Stuff you remember from your high school biology class…
Glucose
Galactose
Lactose
Lactase
Glucose
Galactose +
Lactase
Cells need glucose for cellular respiration.
People who are lactose intolerant don’t have enough lactase to break down lactose.
One option is to take Lactaid. Lactaid is the enzyme lactase that helps lactose intolerant people break down lactose.

12. Testing for Glucose
Glucose test strips indicate presence or absence of glucose in a solution

13. New page: Lactase Lab
Question: How does heating an enzyme affect its function? Data Collection:
Milk
Not boiled Lactase
Boiled Lactase
Glucose
Milk +
lactase
boiled lactase
Starting color
blue
Color at 60 sec
Presence or absence of glucose