1. Method development for the Analysis of Body Odor Using Gas Chromatography-Mass Spectrometer
Revathi a/p Rajan
Postgraduate Student, School of Health Sciences
Masters in Forensic Chemistry
Universiti Sains Malaysia
Main Supervisor : Dr Nik Fakhuruddin Nik Hassan

2. Body Odor
Combination of volatile and semi-volatile organic compounds produced by human skin
Origin of human odor is yet to be discovered.
Three categories : Primary, Secondary and Tertiary

3. Previously employed methods
ATD GCMS
SPME GCMS
Solvent Extraction with GCMS
Purge and Trap GCMS

4. Methods Compared in This Research
Headspace Injections
Liquid Injections
Sample Preparation Optimization
Steam Distillation and LLE (non polar solvent)
Direct Extraction (non polar solvent)
Direct Extraction (polar non polar mixture solvent)
Steam Distillation (polar and non polar mixture solvent)
Direct Extraction (polar solvent)
Qualitative Method Validation
Real Sweat Sample Analysis
PCA Analysis
Analytical parameters optimization
Sample collection optimization
Sample preparation optimization
Collection Material Optimization
Real Sweat Sample Analysis

5. Static Headspace Injection
Inlet Temperature : 200ºC
Maximum Oven Temp : 200ºC
Transfer Line Temp :250ºC
Sample Collection : 60 min
Inlet Temperature : 200ºC
Maximum Oven Temp : 200ºC
Transfer Line Temp :230ºC
Sample Collection : 20 min
Inlet Temperature : 200ºC
Maximum Oven Temp : 200ºC
Transfer Line Temp :230ºC
Sample Collection : 20 min
Inlet Temperature : 200ºC
Maximum Oven Temp : 270ºC
Transfer Line Temp :250ºC
Sample Collection : 45 min
Inlet Temperature : 200ºC
Maximum Oven Temp : 200ºC
Transfer Line Temp :250ºC
Sample Collection : 20 min
Inlet Temperature : 250ºC
Maximum Oven Temp : 280ºC
Transfer Line Temp :280ºC
Sample Collection : 45 min

6. Polar and Non Polar Mixture
Liquid Injection
Direct Extraction
Polar and Non Polar Mixture
Direct Extraction
Polar Solvent

7. Subject Analysis Results
Inter Individual Variation
Intra Individual Variation

8. Conclusion
Direct extraction using methanol as solvent was the optimum method for the analysis of the less volatile component present in the body odor
Study of the odor component revealed that individualization can be achieved using primary odor component and that odor is unique for every individual.

9. Thank you