1. Poster No. 155 L-ASPARAGINASE PRODUCING HALOPHILIC
AND ALKALIPHILIC SALINICOCCUS SP.
BHAT M.R.*, NAIR J.S. & T.MARAR
Padmashree Dr.D.Y.Patil University,Department of Biotechnology & Bioinformatics,
CBD Belapur, Navi Mumbai, Maharashtra, India ,
Poster No. 155
Abstract
L-asparaginase - an effective therapeutic agent against lymphocytic leukemia, also finds applications in food industry
Soil bacteria were screened for L-asp from soil of Tungareshwar, Vasai, MS, India.
Medium used - Modified M9 medium
The characterization of pigment, purified enzyme and anticancer property if any could lead to novel application of the isolate
Total isolates screened - 885
Maximum L-asp producing isolate - WS2
Method - Rapid plate assay and bioassay
No reports of L-asparaginase production from Salinicoccus sp. Cited
Results
Keywords: L-asparaginase, akaliphile, halophile, 16S ribosomal RNA, Salinicoccus sp.
Table no. 1 IMVic Tests & Sugar Utilisation , Table no. 2 ,3 Salt & pH
tolerance
Test
Result
Indol
Negative
M.R.
V.P.
Citrate
Catalase
Positive
Oxidase
Nitrate Reduction
Glucose
No acid
Arabinose
Lactose
Adonitol
Sorbitol
Manitol
Rhanose
Sucrose
Lysinedecarboxylase
Ornithine
Phenylalanine D’nas
H2S pH
Growth 7 + 8 9 10 11 12
References- Images from
medcitynews.com, kianmed.ir 
pinkycraft.blogspot.com
flipper.diff.org,
L-asparaginase – Action & Application
Whats Next 1
Action of L-asparaginase
Salt Con.
Growth 2 + 4 6 8 10 12 4
Applications in FoodIndustry 2 3
Antileukemic brands
Antileukemic action
Discussion
The 16S ribosomal RNA gene sequencing analysis indicated - Salinicoccus NEAU-ST10-44 (99% identity). The isolate was found to be catalase and oxidase positive. No Acid production was observed for carbohydrates. IMViC test were found negative and Nitrate reduction test was found positive for the isolate. The isolate tolerated 12% of salt concentration and grew at pH 12.
Conclusion
The potential isolate under study is a high yielding extracellular L-asparaginase producing gram positive, akalophilic and halophilc and an orange pigment producer. The characterization of pigment, purified enzyme and anticancer property if any could lead to novel application of the isolate.
Purpose
Isolation of L-asparaginase from novel isolates is the need of the hour due to allergic reactions & immunological effects of existing sources of L-asparaginase. Less literature is available on L-asparaginase producing gram positive akalophilic and halophilc bacteria.
References
Methods
Isolation and enumeration- St. Modified M9 agar containing 1% L-asparagine and 0.005% phenol red was used. Enzyme production is accompanied by an increase in pH of the medium, which results in the formation of pink zone. Bioassy / Agar cup method- method was also carried out to screen the potential isol
16s DNA sequencing - 1.
Gulati R, Saxsena RK, Gupta R – 1997, Rapid plate assay for screening of L-Asparaginase producing microorganisms , Letters in applied microbiology, Volume 24, :23-26 2
Verma N, Kumar K, Kaur G, Anand S- 2007, L-asparaginase a promising chemotherapeutic agent, Critical review in Biotechnology, Volume 27:45-62 3
Wriston J.C.Jr. and Yellin T.O.-1973,L- asparaginase - A Review, Advanced Enzymes,Volume 39,
Query length: 1292 bases
Program: BLASTN
ACCESSIONJQ762292
Salinicoccus sp. NEAU-ST10-44
Maximum
99%identity
Acknowledgements:
Thanks to Prof. Dasgupta, head DBB,PDDYPU & Dr. Nair , Prof . T. Marar , Guide for the entire support in the work