1. Development and clinical evaluation of a novel fully automated qualitative PCR assay for the diagnosis of anogenital herpes simplex virus infection 
Steve Miller, Erik Samayoa, Linda Post, Carolyn Wright, Geoff McKinley, Michael Wood, Jesus Ching 
Diagnostic Microbiology and Infectious Disease 
Volume 80, Issue 2, Pages (October 2014)
DOI: /j.diagmicrobio
Copyright © 2014 Elsevier Inc. Terms and Conditions

2. Fig. 1
IDbox HSV 1/2 assay cassette showing reagent and sample reaction chambers. The sample is manually added and cassette placed in the instrument. The instrument automatically adds the internal control, performs proteinase K lysis, and binds magnetic silica beads. The beads are passed to wash chambers using magnetic bead transfer valves, and the purified nucleic acid is added to the PCR reaction chamber, which contains lyophilized primers, probes, and master mix. Real-time PCR proceeds on the instrument, and the signal and re-association curves are determined using fluorescent detectors.
Diagnostic Microbiology and Infectious Disease  , DOI: ( /j.diagmicrobio )
Copyright © 2014 Elsevier Inc. Terms and Conditions

3. Fig. 2
Receiver-operator curves showing comparison of IDbox results to HSV culture (A) and HSV PCR (B).
Diagnostic Microbiology and Infectious Disease  , DOI: ( /j.diagmicrobio )
Copyright © 2014 Elsevier Inc. Terms and Conditions

4. Fig. 3
Comparison of IDbox HSV 1/2 assay re-association peak results with real-time PCR viral load and PCR sequencing. Samples yielding positive sequencing results are shown as boxes, while samples yielding negative sequencing results are shown as diamonds.
Diagnostic Microbiology and Infectious Disease  , DOI: ( /j.diagmicrobio )
Copyright © 2014 Elsevier Inc. Terms and Conditions