1. Kalinat
Anti wrinkle

2. An innovative and Powerful
KALINAT A-W
An innovative and Powerful
ANTI AGE
& ELASTICIZING
Ingredient
KALINAT A-W

3. Sodium salt of deoxyribonucleic acid (DNA) segments
COMPOSITION
COMPOSITION
Sodium salt of deoxyribonucleic acid (DNA) segments
KALINAT A-W
INCI NAME
Sodium DNA
PURITY
Powder
PRODUCTION SCHEME:
DNA Extraction (biotechnologies)
Purification - stabilization
Buffering through sodium ions

4. In Vitro
Test

5. FIBROBLAST STIMULATION
% Fibroblast growth after 24 hours of exposure to different amounts of Kalinat
KALINAT A-W
110 %
SUGGESTED USAGE %
0,1 ÷ 0,25
1,00 %
0,50 %
0,25 %
0,13 %
0,06 %
% Fibroblast growth
100 %
Control

6. 1 – UVA PHOTO TOXICITY Vitality of irradiated fibroblasts
Capacity to protect cells in UVA “stressed” culture
KALINAT A-W
UVA
UVA
DEcrease of
the
cellular
vitality
Fibroblasts + Kalinat
INcrease of the cellular vitality,
Fibroblasts

7. In
Test
Vivo

8. IN VIVO INSTRUMENTAL EVALUATION
Emulsion Kalinat® @ 0,25% vs. placebo
20 subjects,
Average age 49
8 weeks Two application par day
Right & Left peri-ocular area
KALINAT A-W

9. IN VIVO TESTS Moisture level (Corneometer C&K)
Cutaneous thickness (Dermascan C Ver3)
Elasticity (Cutometer C&K)
Maximum Roughness (Skin replica)
Skin replica pictures
KALINAT A-W

10. 1 – MOISTURE LEVEL + 5% + 0% % of increase of moisture levol 64 % 62 %
KALINAT A-W
64 %
62 %
% of increase of moisture levol
60 %
Week # 0
Week # 8
Week # 0
Week # 8
with Kalinat AW
with Placebo
+ 5% %

11. 2 – CUTANEUS THICKNESS + 8,7% vs + 2%
KALINAT A-W
1,50
1,43
Week # 0
Week # 8
1,39
Cutaneus Thickness (mm)
with Kalinat AW
with Placebo
+ 8,7% vs + 2%

12. 3 – ELASTICITY + 25,6% vs < 0%
VERY HIGH
KALINAT A-W
0,49
0,38
0,35
Week # 0
Week # 8
Elasticity
with Kalinat AW
with Placebo
+ 25,6% vs < 0%

13. 4 – MAXIMUM ROUGHNESS - 4% vs - 1,9%
KALINAT A-W
157,7
154,9
151,4
Max.Rugosity (µm)
Week # 0
Week # 8
with Kalinat AW
with Placebo
- 4% vs - 1,9%

14. 5 – SKIN REPLICA PICTURES BEFORE AFTER
KALINAT A-W
BEFORE
AFTER
Week # 0
Week # 8

15. In Vitro test Very Important fibroblast stimulation
CONCLUSION
Very Important fibroblast stimulation
No cytotoxic or photo-toxic effects
Protective and regenerative action on the epidermis
KALINAT A-W

16. In Vivo test + 5% + 9% Moisture level Cutaneous thickness + 25%
CONCLUSION
+ 5%
+ 9%
+ 25%
- 4%
KALINAT A-W
Moisture level
Cutaneous thickness
Elasticity
Maximum Roughness

17. Ingredient that can be used in…
THUS…
a very powerful
ANTI AGE
& ELASTICIZING
Ingredient that can be used in…
KALINAT A-W

18. SUITABLE FOR Sun care products (sunscreen creams and milk, after sun)
Anti-age & Anti-wrinkle face cream
Sun care products (sunscreen creams and milk, after sun)
Baby products (protective, soothing)
Treatments for dry skins
Treatment for damaged skins (i.e. stretch marks)
KALINAT A-W

19. Via Pastore 1 – 25082 (Brescia) Italy
Thank you
KALINAT A-W
Via Pastore 1 – (Brescia) Italy
Tel Fax