1. Donor-derived bone marrow transfusion produces mixed chimerism and promotes a Th2 shift in Th1/Th2 balance in rat heterotopic small bowel transplantation 
Anbin Hu, Qiang Li, Huijuan Shi, Qiang Tai, Linwei Wu, Jun Xiong, Bimang Fu, Xiaoshun He 
Digestive and Liver Disease 
Volume 44, Issue 12, Pages (December 2012)
DOI: /j.dld
Copyright © 2012 Editrice Gastroenterologica Italiana S.r.l. Terms and Conditions

2. Fig. 1
Mix chimerism is induced by simultaneous donor derived bone marrow transfusion with small bowel transplantation (SBTx) in rats by real-time PCR analysis. Real-time PCR analysis for sex-determining region of Y chromosome (SRY) in various female Lewis recipient tissues (blood, liver, spleen and intestine) at day 60 after received male BN intestinal grafts in control group, tacrolimus (FK506) group, and SBTx plus oral FK506 and intraportal infusion of donor-derived bone marrow cells (BM-PV) group. Data were expressed as the mean±SD (*p<0.05, control group vs BM-PV group; #p<0.05, FK506 group vs BM-PV group; n=5).
Digestive and Liver Disease  , DOI: ( /j.dld )
Copyright © 2012 Editrice Gastroenterologica Italiana S.r.l. Terms and Conditions

3. Fig. 2
Mix chimerism is induced by simultaneous donor derived bone marrow transfusion with small bowel transplantation (SBTx) in rats by fluorescence in situ hybridization (FISH) analysis. FISH analysis for sex-determining region of Y chromosome (SRY) in various female Lewis recipient tissues (liver, spleen and intestine) at day 60 after received male BN intestinal grafts in control group, tacrolimus (FK506) group, and SBTx plus oral FK506 and intraportal infusion of donor-derived bone marrow cells (BM-PV) group. Green fluorescence represents SRY positive signal. White arrows indicate cells derived from male BN rats in recipient liver and spleen (original magnification ×100). Data were expressed as the mean±SD (*p<0.05, control group vs BM-PV group; #p<0.05, FK506 group vs BM-PV group; n=5).
Digestive and Liver Disease  , DOI: ( /j.dld )
Copyright © 2012 Editrice Gastroenterologica Italiana S.r.l. Terms and Conditions

4. Fig. 3
Survival curve for simultaneous donor derived bone marrow transfusion with small bowel transplantation (SBTx) in rats. Recipient (control group, tacrolimus (FK506) group, and SBTx plus oral FK506 and intraportal infusion of donor-derived bone marrow cells (BM-PV) group) survival was monitored after SBTx (p=0.006, FK506 group vs control group; p= , FK506 group vs BM-PV group; n=10).
Digestive and Liver Disease  , DOI: ( /j.dld )
Copyright © 2012 Editrice Gastroenterologica Italiana S.r.l. Terms and Conditions

5. Fig. 4
Histological changes in intestinal grafts after small bowel transplantation (SBTx) in rats. (A) The intestinal graft tissues were collected on day 7, 14, 30 and 60 after SBTx in control group, tacrolimus (FK506) group, and SBTx plus oral FK506 and intraportal infusion of donor-derived bone marrow cells (BM-PV) group. Haematoxylin–eosin staining was used for histological analysis (original magnification ×100). (B) Small bowel pathology score were statistical analysis from panel (A). The evaluation of the histological grade of injuries was based on three parameters from 0 to 2: the percentage of injured villi, the relative villus height within the mucosa, and the total mucosal thickness. Data were expressed as the mean±SD (*p<0.05, vs control group; #p<0.05, vs FK506 group; n=10).
Digestive and Liver Disease  , DOI: ( /j.dld )
Copyright © 2012 Editrice Gastroenterologica Italiana S.r.l. Terms and Conditions

6. Fig. 5
Rejection grading analysis of intestinal grafts after small bowel transplantation (SBTx) in rats. (A) Criterion of rejection grade in SBTx (normal=0; mild, moderate and severe acute rejection=1, 2, and 3, respectively; chronic rejection=4). (B) Rejection score of recipient in control group, tacrolimus (FK506) group, and SBTx plus oral FK506 and intraportal infusion of donor-derived bone marrow cells (BM-PV) group were measured on day 0, 7, 14, 30 and 60. Data were expressed as the mean±SD (*p<0.05, compared with control group; #p<0.05, compared with FK506 group; n=5).
Digestive and Liver Disease  , DOI: ( /j.dld )
Copyright © 2012 Editrice Gastroenterologica Italiana S.r.l. Terms and Conditions

7. Fig. 6
Changes of serum IL-2 and IL-10 levels after small bowel transplantation (SBTx) in rats. (A and B) Enzyme labelled immunosorbent assay (ELISA) was used to monitor serum IL-2 and IL-10 after SBTx in control group, tacrolimus (FK506) group, and SBTx plus oral FK506 and intraportal infusion of donor-derived bone marrow cells (BM-PV) group recipient on day 0, 7, 14, 21, 30 and 60 after SBTx. (C) Changes of IL-2/IL-10 ratio were figured out by panel A and B. Data were expressed as the mean±SD (*p<0.05, compared with control group; #p<0.05, compared with FK506 group; n=5).
Digestive and Liver Disease  , DOI: ( /j.dld )
Copyright © 2012 Editrice Gastroenterologica Italiana S.r.l. Terms and Conditions