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Plant Lipids Peter Dörmann

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1 Plant Lipids Peter Dörmann
Institute of Molecular Physiology and Biotechnology (IMBIO) Bonn: since February 2008 MPI Golm: until January 2008

2 Lipids are essential for photosynthesis
DGDG (%) WT dgd1

3 Storage lipids in seeds: human nutrition and biofuel production
Plant Oil Salade oil Oil body in the cytosol of plant cells Biodiesel

4 Plant lipids and plant microbe interactions
N2 -> NH4+ Lotus japonicus Lotus nodules infection Rhizobium thread Plant galactolipids are required for nodulation Mesorhizobium loti

5 Galactolipids replaces phospholipids during phosphate limitation
R-P- nucleic acids phosphoproteins sugar-phosphates Phospholipids DGDG - P Pi deficiency Pi - P

6 The lipid response to abiotic stress: phosphate deprivation
Arabidopsis thaliana Lotus japoncius Total Lipid Extract Lipid measurements: Targeted approaches for defined lipid classes Non-targeted approaches for novel and unknown lipids

7 Targeted lipid analysis
Phosphatidylcholine Phosphatidylethanolamine Phosphatidylglycerol Phosphatidylserine Phosphatidylinositol Phosphatidic acid Monogalactosyldiacylglycerol Diagalactosyldiacylglycerol Sufolipid Ceramide Glucosylceramide Free Sterol, Sterol ester Sterol glucoside Acylated sterol glucoside Diacylglycerol Triacylglycerol Wax ester Tocopherol, Plastoquinol, Phylloquinol Targeted lipid analysis Lipid LC-MS (Q-TOF) GC-MS In total: More than 300 molecular species

8 Nont-targeted screening for stress-dependent lipids
Example: TOF lipid mass spectra of roots versus nodules Roots Ca lipid peaks each Nodules 21 masses > 50 x increased masses > 20 x increased masses > 10 x increased masses > 5 x increased masses > 2 x increased Characterization and identification of compounds by fragmentation

9 Aims: Record lipid changes during phoshpate deprivation
Time courses: early (lipid signalling molecules), late changes Changes in lipid intermediates and byproducts: diacylglycerol, triacylglycerol Arabidopsis lipid mutants Include lipids not been previously measured (Glycosylinositolphosphoceramides) Plant species: Arabidopsis, Lotus japonicus (legume), barley (monocot) -OH

10 Outloock Abiotic stresses: cold stress, freezing stress Senescence
Additional plant species...

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