1. Lab session 1

2. Media preparation
Media sterilization
Use and care of the microscope

3. Media – nutrients
Used to grow microbes
Glucose – energy
Peptones – breakdown products of protein
Good source of nitrogen, amino acids
Yeast extract - vitamins

4. Liquid medium – nutrient broth
Large amount of culture
Solid medium – nutrient agar
Agar – solidifying agent
Extracted from red algae
Polysaccharide

5. Granules of agar from a commercial source
Add that to nutrient broth
Heat the mixture to dissolve granules
Solution cools down to 40oC agar becomes a solid

6. Agar – good solidifying agent
Microbes cannot breakdown agar
Cannot metabolize
No nutritional value
Stays a solid even in incubators
Study colony morphology
Isolate a specific species from a sample

7. Agar – sterilized in the autoclave
Temperature 121oC, pressure 15psi, 15 minutes
Heat labile solutions – solutions of enzymes
Filtration
Microbes stay on the surface of filter
Solution free of microbes goes through the filter

8. Dry sterilization
Glassware – pipets
Oven – 180oC for 2 hours
Chemical sterilization
Disinfectant –clean table tops

9. Microscope Use of oil immersion objective Focusing an object
Total magnification
Cleaning and storing