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In Situ Detection of Hepatitis C Virus RNA in Salivary Glands

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Presentation on theme: "In Situ Detection of Hepatitis C Virus RNA in Salivary Glands"— Presentation transcript:

1 In Situ Detection of Hepatitis C Virus RNA in Salivary Glands
Juan José Arrieta, Elena Rodríguez-Iñigo, Nuria Ortiz-Movilla, Javier Bartolomé, Margarita Pardo, Félix Manzarbeitia, Horacio Oliva, David Mariscal Macías, Vicente Carreño  The American Journal of Pathology  Volume 158, Issue 1, Pages (January 2001) DOI: /S (10) Copyright © 2001 American Society for Investigative Pathology Terms and Conditions

2 Figure 1 In situ hybridization in salivary gland biopsies from an anti-HCV-positive (a) and -negative (b) patients. Original magnification, ×400. Intracellular localization of the hybridization signal (c). Original magnification, ×1,000. Counterstained with safranine. The American Journal of Pathology  , DOI: ( /S (10) ) Copyright © 2001 American Society for Investigative Pathology Terms and Conditions

3 Figure 2 In situ hybridization of serial sections of the same salivary gland biopsy with (a) and without (b) predigestion with RNase and hybridized in the presence (c) or in the absence (d) of an excess of unlabeled probe. Original magnification, ×1,000. Counterstained with safranine. The American Journal of Pathology  , DOI: ( /S (10) ) Copyright © 2001 American Society for Investigative Pathology Terms and Conditions

4 Figure 3 In situ detection of HCV-RNA of sense (a) and antisense (b) polarity. Original magnification, ×400. Counterstained with safranine. The American Journal of Pathology  , DOI: ( /S (10) ) Copyright © 2001 American Society for Investigative Pathology Terms and Conditions

5 Figure 4 Dot blot hybridization of serial dilutions of synthetic HCV-RNA of genomic and antigenomic polarity with the HCV antisense (a) and sense (b) probes. The American Journal of Pathology  , DOI: ( /S (10) ) Copyright © 2001 American Society for Investigative Pathology Terms and Conditions

6 Figure 5 Immunological detection of HCV core antigen in salivary gland biopsies from a HCV-RNA-positive (a) and -negative (b) patients. Arrows show the positive acini for the HCV core protein. The American Journal of Pathology  , DOI: ( /S (10) ) Copyright © 2001 American Society for Investigative Pathology Terms and Conditions

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