1. Assessment of Liver Tissue Metabolic State for Transplant using Optical Imaging
by: Shima Mehrvar, Mette la Cour
Advisor: Dr. Ranji
Collaborator: Dr. Kim, Dr. Hong, and Dr. Camara

2. Introduction Motivation Aim
Only 72 % of people who get liver transplant survive after 3 years.
Blood assays does not provide enough functional information about the liver tissue for transplantation.
Metabolic state is an important key marker for hepatic functionality.
Aim
To quantify the metabolic state of livers before, during and after transplant using optical imaging technique.

3. Method
Sample Preparation
Control
Ischemia
Reperfusion

4. Method Cryo imager 3D images
Mitochondrial autofluorescence signals: NADH and FAD
Redox Ratio (RR) = NADH/FAD Metabolic quantitative marker

5. Results
Optical images

6. Results *

7. Future Direction Cryoimager Fluorometry
Can be translated to biopsies from human liver
Further assessment of Ischemia reperfusion injury
Fluorometry
Ex vivo or in vivo probing autofluorescence signals for clinical applications