1. Heparanase procoagulant activity as a predictor of wound necrosis following diabetic foot amputation 
Eli Peled, Eyal Melamed, Tali Banker Portal, Elena Axelman, Doron Norman, Benjamin Brenner, Yona Nadir 
Thrombosis Research 
Volume 139, Pages (March 2016)
DOI: /j.thromres
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2. Fig. 1
Skin tissue from the proximal surgery edge was not predictive to necrosis. The skin tissue samples of 10 patients with necrosis and 10 patients without necrosis were stained. Areas of thrombi (A. ×10, B. ×40, H&E) were seen (arrows). Heparanase (C. ×10, D. ×40) and TFPI (E. ×10, F. ×40) were present in the blood vessels wall and inside the lumen (arrows), while TF (G. ×10, H. ×40) was present mainly in the vessels wall (arrows). Staining did not differ between cases with necrosis and those without necrosis in term of the number of cases with thrombi, pattern or strength of staining. Images were visualized through a x/0.82 MDC objective lens, captured with a Nikon E995 digital camera (Nikon, Tokyo, Japan).
Thrombosis Research  , DOI: ( /j.thromres )
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3. Fig. 2
Heparanase procoagulant activity significantly differentiates between non-necrotic and necrotic cases. The study group included 22 non-necrotic cases and 18 necrotic cases. Blood samples were drawn immediately before the amputation surgery (1), 1h after the surgery (2), 1week post-surgery (3) and 1month post-surgery (4). The most significant changes were observed in the heparanase procoagulant activity 1h post-surgery. Increase in heparanase procoagulant activity was observed in 20/22 non-necrotic cases, while a decrease or no change was observed in all necrotic cases. *P<0.05, **P<0.001, ***P< Results represent mean±SEM.
Thrombosis Research  , DOI: ( /j.thromres )
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4. Fig. 3
Pre-surgery TF+heparanase activity, TF activity, heparanase procoagulant activity and heparanase level in non-necrotic vs. necrotic cases. The first plasma sample (pre-surgery) was studied in non-necrotic (n=22) and necrotic (n=18) cases. Box plots show the changes of parameters. Central horizontal bars, columns, and error bars indicate the median, 25th to 75th percentile, and 10th to 90th percentile, respectively. Although all parameters demonstrated a trend to increase in the necrotic group compared to the non-necrotic group, results were statistically significant only in TF activity.
Thrombosis Research  , DOI: ( /j.thromres )
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