1. Chapter 19:
The Cell Cycle

2. The Cell Cycle

4. The Cell Cycle Control System

5. Cell Cycle Control Molecules
Cyclin-Dependent Kinases (Cdks)
Maturation promotion factor (MPF)
Anaphase-promoting complex (APC)
Rb Protein

6. Cdks
Remember: Phosphorylation/dephosphorylation are commonly used for regulation of protein (enzyme) activity
Cdks: kinases that are only active when bound to a protein from the cyclin family
Several dozen cyclins have been discovered
Their concentrations within a cell increase and decrease throughout the cell cycle

7. Cyclins
Wikipedia.org image

8. Cell Cycle Control Molecules
Cyclin-Dependent Kinases (Cdks)
Maturation promotion factor (MPF)
Anaphase-promoting complex (APC)
Rb Protein

9. Evidence for Existence of MPF

10. MPF Regulation

11. MPF Levels Fluctuate During the Cell Cycle

12. Cell Cycle Control Molecules
Cyclin-Dependent Kinases (Cdks)
Maturation promotion factor (MPF)
Anaphase-promoting complex (APC)
Rb Protein

13. Anaphase-Promoting Complex

14. Cell Cycle Control Molecules
Cyclin-Dependent Kinases (Cdks)
Maturation promotion factor (MPF)
Anaphase-promoting complex (APC)
Rb Protein

15. Rb Protein

16. Cell Cycle Checkpoints
Mitotic spindle checkpoint
DNA Replication checkpoint
DNA Damage checkpoint

17. Mitotic Spindle Checkpoint

19. Cell Cycle Checkpoints
Mitotic spindle checkpoint
DNA Replication checkpoint
DNA Damage checkpoint

20. DNA Damage Checkpoint

21. Putting it all together!

22. Putting it all together!