1. Mallahalli S. Manu, Kuruvanthe S. Rachana, Gopal M. Advirao
Altered expression of IRS2 and GRB2 in demyelination of peripheral neurons: Implications in diabetic neuropathy 
Mallahalli S. Manu, Kuruvanthe S. Rachana, Gopal M. Advirao 
Neuropeptides 
Volume 62, Pages (April 2017)
DOI: /j.npep
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2. Fig. 1
Alteration in the blood glucose levels of normal control, STZ and insulin treated STZ rats. Error bars represent±SEM (n=6).
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3. Fig. 2
Histological examination of tolidine blue stained longitudinal section of sciatic nerve showing the structural difference between 4, 8 and 12week sample of normal[(control) A, B, C] diabetic[(STZ) D, E, F] and insulin treated diabetic samples[(STZ+I) G, H, I]. Arrow heads showing deformation in nerve fiber organization. Scale bar: 50μm.
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4. Fig. 3
Differential expression of pIRS1 protein and IRS1 mRNA level at 4, 8 and 12week sciatic nerve samples correlated between control (C), diabetic (STZ) and insulin treated (STZ+I) and 12h and 24h of the SCs grown in normal medium (C), 40mM glucose (H-GLU) and 10nM insulin added 40mM glucose medium (H-GLU+I). (A and B) Western blot analysis showing relative fold change in pIRS1 protein expression of sciatic nerve in STZ and STZ+I compared to C. (C and D) Western blot analysis showing relative fold change in pIRS1 protein expression of SCs in H-GLU and H-GLU+I compared to C. (E) Real time PCR studies showing change in relative mRNA expression among STZ and STZ+I of sciatic nerve. (F) Real time PCR studies showing change in relative mRNA expression among H-GLU and H-GLU+I of SCs. * indicates p<0.05.
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5. Fig. 4
Immunofluorescence staining for evaluation of pIRS1 protein expression difference among SCs grown for 12h and 24h in normal medium[(control)A and B], high glucose medium[(40mM glucose) C and D] and insulin added high glucose medium[(40mM glucose+10nM insulin) E and F]. Scale bar: 50μm.
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6. Fig. 5
Relative mRNA expression of IRS2 (A) IRS2 mRNA expression of 4, 8 and 12week sciatic nerve of control (C), diabetic (STZ) and insulin treated diabetic rats (STZ+I). (B) Relative mRNA expression of IRS2 among SCs grown in 40mM glucose (H-GLU) and 10nM insulin added 40mM glucose medium (H-GLU+I) at 12h and 24h time interval * indicates p<0.05.
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7. Fig. 6
Western blot analysis of pS(731)IRS2 in sciatic nerve and SC. (A and B) Expression of pS(731)IRS2 protein of 4, 8 and 12week sciatic nerve samples correlated between control (C), diabetic (STZ) and insulin treated diabetic rats (STZ+I). (B and C) Relative fold change of pS(731)IRS2 at 12h and 24h of the SCs grown in normal medium (C), 40mM glucose (H-GLU) and 10nM insulin added 40mM glucose medium (H-GLU+I). * indicates p<0.05.
Neuropeptides  , 71-79DOI: ( /j.npep )
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8. Fig. 7
Immunofluorescence staining for evaluation of pS(731)IRS2 protein expression difference among SCs grown for 12h and 24h in normal medium [(control) A and B], high glucose medium [(40mM glucose) C and D] and insulin added high glucose medium [(40mM glucose+10nM insulin) E and F]. Scale bar: 50μm.
Neuropeptides  , 71-79DOI: ( /j.npep )
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9. Fig. 8
Differential expression of pGRB2 protein and GRB2 mRNA level at 4, 8 and 12week sciatic nerve samples correlated between control (C), diabetic (STZ) and insulin treated diabetic rats (STZ+I). 12h and 24h of the SCs grown in normal medium (C), 40mM glucose (H-GLU) and 10nM insulin added 40mM glucose medium (H-GLU+I). (A and B) Western blot analysis showing relative fold change in pGRB2 protein expression of sciatic nerve in STZ and STZ+I compared to C. (C and D) Western blot analysis showing relative fold change in pGRB2 protein expression of SCs in H-GLU and H-GLU+I compared to C. (E) Real time PCR studies showing change in relative mRNA expression of GRB2 among STZ and STZ+I of sciatic nerve. (F) Real time PCR studies showing change in relative mRNA expression of GRB2 among H-GLU and H-GLU+I of SCs. * indicates p<0.05.
Neuropeptides  , 71-79DOI: ( /j.npep )
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10. Fig. 9
Immunofluorescence staining for evaluation of pGRB2 protein expression difference among SCs grown for 12h and 24h in normal medium [(control) A and B], high glucose medium [(40mM glucose) C and D] and insulin added high glucose medium [(40mM glucose+10nM insulin) E and F]. Scale bar: 50μm.
Neuropeptides  , 71-79DOI: ( /j.npep )
Copyright © 2016 Elsevier Ltd Terms and Conditions

11. Neuropeptides 2017 62, 71-79DOI: (10.1016/j.npep.2016.12.004)
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