1. Skyline not identifying peptides
Eric Mbua

2. Transition setting:

3. Peptide sequence: LSELVQAVSDPSSPQYGK found in protein not identified by Skyline

4. LSELVQAVSDPSSPQYGK: Xcalibur
precursor
ion (Light)
Fragment
ion(Light)
ion(Heavy)
Peptide not identified on Skyline when raw data was imported

5. Peptide sequence: LASVLGSEPSLDSEVTSK found in protein not identified by Skyline

6. LASVLGSEPSLDSEVTSK: Xcalibur
precursor
ion (Light)
Fragment
ion(Light)
ion(Heavy)
Peptide not identified on Skyline when raw data was imported

7. Peptide: YYEEFPINLK
No chromatograms for the above peptide when raw data was imported on to Skyline

8. Peptide: YYEEFPINLK: Xcalibur
precursor
ion (Light)
Fragment
ion(Light)
precursor
ion(Heavy)
Fragment
ion(Heavy)
Heavy labeled peptide observed using Xcalibur; but not when raw data was
imported on to skyline