1. Prechill vs. Non Prechill
Kentucky Bluegrass
Prechill vs. Non Prechill

2. AOSA Rules Petri dish 15C – 25C Light Preliminary Count 10 days
Final Count 21 days
Potassium Nitrate
Prechill at 10C for 5 days

3. Objective of the Referee
Comparing germination percentage between prechill and non prechill
Whether one method has an advantage over the other
To survey the uniformity among participant laboratories

4. Sample A
Was harvest in August 2013
Claimed 85% Germination

5. Sample A
Week 1

6. Week 2

7. Final Week

8. Sample B
Was harvested in August 2012
Claimed 85% Germination

9. B Sample Results
Week 1

10. Week 2

11. Final Week

12. Sample C
Was harvested in December 2010
Claimed 80% Germination

13. C Sample Results
Week 1

14. Week 2

15. Final Week

16. Combined Data Results There were 16 total participants
Result is the average of all the participants data for each sample
Result was compared between prechilled vs non prechilled samples

17. Prechill vs Non Prechill

18. Analysis df Mean F Source of variation Square Value Prob
A N A L Y S I S O F V A R I A N C E T A B L E
df Mean F
Source of variation Square Value Prob
Labs (L) ***
Seed Lots (SL) ***
Chill va no-chill (C) ns
(SL) x (C) ns
Error
Coefficient of Variation: 3.78%

19. Conclusions
Noticed higher germination percentages during the first week for prechilled samples
Prechilling treatment did not have significant impact on the germination percentage

20. Special Thank You Thank you to all participants
Dr. Elias Sabry from Oregon State University for data analysis
Seedway LLC, especially Jason Perrault for providing the samples
Nishit Patel from PA Seed Laboratory for putting the samples together.