1. Equine Multinodular Pulmonary Fibrosis and Lymphoma in a Horse Associated with Equine Herpesvirus-5 
Bhupinder Bawa, DVM, PhD, DACVP, Karie Vander Werf, DVM, Laurie Beard, DVM, MS, DACVIM, Elizabeth Davis, DVM, PhD, DACVIM, Gordon Andrews, DVM, PhD, DACVP, Kelli Almes, DVM, DACVP 
Journal of Equine Veterinary Science 
Volume 34, Issue 5, Pages (May 2014)
DOI: /j.jevs
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2. Fig. 1
Lung; horse. The lung parenchyma contained multiple, randomly scattered, variably sized, well-delineated nodules predominantly present in the caudodorsal lung area (arrows).
Journal of Equine Veterinary Science  , DOI: ( /j.jevs )
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3. Fig. 2
Lung; horse. On cut section, the nodule was well circumscribed and tan and compressed the adjacent normal pulmonary parenchyma.
Journal of Equine Veterinary Science  , DOI: ( /j.jevs )
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4. Fig. 3
Spleen; horse. The spleen was diffusely enlarged, and the adjacent splenic lymph nodes were markedly enlarged.
Journal of Equine Veterinary Science  , DOI: ( /j.jevs )
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5. Fig. 4
Lymph node; horse. The chain of sublumbar lymph nodes was diffusely enlarged.
Journal of Equine Veterinary Science  , DOI: ( /j.jevs )
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6. Fig. 5
Lymph node; horse. The enlarged lymph nodes were pale, tan, and bulged on cut sections.
Journal of Equine Veterinary Science  , DOI: ( /j.jevs )
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7. Fig. 6
Heart; horse. Diffusely the subendocardial surface of the left ventricle, left atrium, and multifocally the right ventricle (not shown in the figure) are multifocally expanded by white, firm, gritty areas of mineralization. There was marked subintimal mineralization of the aorta (arrow) and the pulmonary artery.
Journal of Equine Veterinary Science  , DOI: ( /j.jevs )
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8. Fig. 7
Lung; horse. The photomicrograph shows the pulmonary nodule at lower magnification. Note that the nodule is expansile and well delineated and compresses the adjacent normal pulmonary parenchyma on the right side (arrows). Hematoxylin and eosin stain.
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9. Fig. 8
Lung; horse. On higher magnification, the nodule is composed of alveolar architecture with interstitium broadly expanded by fibrous connective tissue and collaged bands. The alveolar lumen is filled with eosinophilic proteinaceous fluid mixed with macrophages, and fewer neutrophils. A focal area of mineralization (arrow) is present. Hematoxylin and eosin stain.
Journal of Equine Veterinary Science  , DOI: ( /j.jevs )
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10. Fig. 9
Lung; horse. Note the alveolar interstitium is expanded by abundant fibrous connective tissue stained blue. Masson's trichrome stain and aniline blue counterstain.
Journal of Equine Veterinary Science  , DOI: ( /j.jevs )
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11. Fig. 10
Lymph node; horse. All the lymph nodes in the body were enlarged and microscopically the lymph node architecture is replaced by sheets of round neoplastic cells. Two populations of cells were seen, one most predominant population composed of small mature lymphocytes, and the other smaller population was composed of large lymphocytes. Scattered in the neoplastic cells were large numbers of multinucleated giant cells (arrow). Inset: Higher magnification of multinucleated giant cells showing eosinophilic needle-like crystalline structures (arrowhead). Hematoxylin and eosin stain.
Journal of Equine Veterinary Science  , DOI: ( /j.jevs )
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12. Fig. 11
Lymph node; horse. Most cells in the lymph node were small and mature and stained positive with CD3 immunostain, confirming T-cell lineage. CD3 stain and hematoxylin counterstain.
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13. Fig. 12
Lymph node; horse. The larger appearing and fewer cells in lymph node were positive for CD20 and CD79a (not shown). CD20 stain and Hematoxylin counterstain.
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14. Fig. 13
The Lymph node and lung fresh tissue were submitted for equine herpesvirus-5 glycoprotein H polymerase chain reaction. Lane 1 = 100-bp DNA ladder; lane 2 = lymph node; lane 3 = lung; lane 4 = EHV-5-positive control; lane 5 = negative control.
Journal of Equine Veterinary Science  , DOI: ( /j.jevs )
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