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Morphological and molecular differences in corpus luteum of pregnant sows from divergent genetic groups  Karine A. Costa, Walmir da Silva, Renata Veroneze,

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Presentation on theme: "Morphological and molecular differences in corpus luteum of pregnant sows from divergent genetic groups  Karine A. Costa, Walmir da Silva, Renata Veroneze,"— Presentation transcript:

1 Morphological and molecular differences in corpus luteum of pregnant sows from divergent genetic groups  Karine A. Costa, Walmir da Silva, Renata Veroneze, José C. Montes, Lucas L. Verardo, Margareth E. Botelho, Márcio S. Duarte, Mariana M. Neves, Paulo S. Lopes, José D. Guimarães, Susana A. Teixeira, Laene Alcantara, Simone E.F. Guimarães  Theriogenology  Volume 104, Pages (December 2017) DOI: /j.theriogenology Copyright © 2017 Elsevier Inc. Terms and Conditions

2 Fig. 1 Corpus luteum vascularization. Blood Vessels/capillaries mean at seven, 15, 30, 45, 60 and 90 gestational ages in corpus luteum of pregnant sows. Different letters represent significant differences between the averages of blood vessels/capillaries number in corpus luteum by Tukey test (P = 0.05). Theriogenology  , DOI: ( /j.theriogenology ) Copyright © 2017 Elsevier Inc. Terms and Conditions

3 Fig. 2 Expression of angiogenesis genes. Fold change of (a) MMP9, (b) VEGFA, (d) ANGPT1 expression across gestational ages of corpus luteum of local Piau breed (LPB) and Commercial line (COM). (c) Fold change of ANGPT1 between genetics groups (COM and LPB) throughout pregnancy. *P = 0.05. Theriogenology  , DOI: ( /j.theriogenology ) Copyright © 2017 Elsevier Inc. Terms and Conditions

4 Fig. 3 Angiopoietins ratio during pregnancy. ANGPT2/ANGPT1 ratio in (a) Commercial line and (b) local Piau breed corpus luteum throughout pregnancy. *P = 0.05. Theriogenology  , DOI: ( /j.theriogenology ) Copyright © 2017 Elsevier Inc. Terms and Conditions

5 Fig. 4 Expression of apoptotic genes. Fold change of (a) BAX, (c) BCL2 and (e) CASP3 expression in corpus luteum across gestational ages of local Piau breed (LPB) and Commercial line (COM). Fold change of (b) BAX, (d) BCL2 and (f) CASP3 expression between genetics groups (COM and LPB) throughout pregnancy. *P = 0.05. Theriogenology  , DOI: ( /j.theriogenology ) Copyright © 2017 Elsevier Inc. Terms and Conditions

6 Fig. 5 Heatmap of corpus luteum angiogenesis and apoptosis. Heatmap of (a) angiogenic and (b) apoptotic genes in Commercial (COM) and Piau (LPB) corpus luteum during pregnancy. Unsupervised hierarchical clustering of angiogenic and apoptotic differentially expressed genes across six gestational ages (7, 15, 30, 45, 60 and 90 days of pregnancy). Angiogenic genes were divided in five groups by similar expression pattern. Apoptotic genes were clustered in only one group by similar expression pattern. A median expression value equals to zero was designated in gray; black means reduced expression and white increased expression. Theriogenology  , DOI: ( /j.theriogenology ) Copyright © 2017 Elsevier Inc. Terms and Conditions

7 Fig. 6 Steroidogenic enzymes abudance. Protein abundance of CYP11A1, HSD3B1 and β-TUBULIN in corpus luteum at seven, 15, 30, 45, 60 and 90 days of gestation in local Piau breed (LPB) and Commercial (COM) females. Theriogenology  , DOI: ( /j.theriogenology ) Copyright © 2017 Elsevier Inc. Terms and Conditions


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