1. The Value of Optical Coherence Tomography in Glioma Surgery
David Jaehyun Park, MD, PhD¹, Jae Sung Park, MD¹, Ji Sun Woo, PhD2,
Seong Muk Kim, PhD2, Yong Joon Joo, PhD3, Tae Joong Eom,PhD3, Sin-Soo Jeun, MD, PhD¹
¹Department of Neurosurgery, Seoul St. Mary's Hospital, The Catholic University of Korea, Seoul, Korea
2Department of Biomedical Science, College of Medicine, The Catholic University of Korea, Seoul, Korea
3Advanced Photonics Research Institute, Gwangju Institute of Science and Technology, Gwangju, Korea
David Jaehyun Park, MD, PhD
Department of Neurosurgery, Seoul St. Mary's Hospital, The Catholic University of Korea,
222 Banpo-daero, Seocho-gu, Seoul, , Korea.
Tel: , Fax: ,
The Value of Optical Coherence Tomography in Glioma Surgery Slide 1 of 12

2. Introduction Image-guided surgery Functional neuroimaging
To maximize the extent of tumor resection
& minimize the surgical morbidity
Image-guided surgery
Intraoperative imaging
Introduction
A number of different methods to localize a glioma intraoperatively have been introduced. Neuronavigation, intraoperative MRI, Intraoperative sonography, and 5-aminolevulinic acid are those methods. As brain shifts after opening dura, not only localization but also detection of residual tumor have an important role in glioma surgery.
Functional neuroimaging
Intraoperative brain mapping
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3. Introduction Preoperative imaging preop navigation image Tumor at
beginning of surgery
preop navigation image
Introduction
A number of different methods to localize a glioma intraoperatively have been introduced. Neuronavigation, intraoperative MRI, Intraoperative sonography, and 5-aminolevulinic acid are those methods. As brain shifts after opening dura, not only localization but also detection of residual tumor have an important role in glioma surgery.
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4. Intraoperative imaging
Introduction
Preoperative imaging
Intraoperative imaging
Tomographic imaging
Biological imaging
Introduction
A number of different methods to localize a glioma intraoperatively have been introduced. Neuronavigation, intraoperative MRI, Intraoperative sonography, and 5-aminolevulinic acid are those methods. As brain shifts after opening dura, not only localization but also detection of residual tumor have an important role in glioma surgery.
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5. Optical Coherence Tomography
Purpose
Optical Coherence Tomography
Purpose
In the present study, we focused on optical coherence tomography (OCT). OCT is a non-invasive and non-contact imaging technology. It allows discrimination of normal brain, solid tumor tissue, and diffusely invaded brain tumor tissue. OCT imaging allowed acquisition of 2D and 3D data arrays for multi-planar analysis of the tumor to brain interface.
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6. Material & Methods 150 mm Handheld OCT imaging probe
In this study, we made U87-Luc human glioma cell xenograft mouse models to check the intraoperative usefulness of OCT imaging in glioma surgery. To extend OCT imaging depth, we developed an OCT system with 1300 nm wavelength range. The OCT system enhanced image contrast difference between gliomas and normal brain tissue. Fluorescence imaging and OCT imaging were done and accuracy of the images was analyzed. Moreover, hematoxylin and eosin (H&E) staining was done for each xenograft mouse model to confirm the margin of glioma. Both ex-vivo and in-vivo experiments were performed.
Handheld OCT imaging probe
Size : 150x50x30 mm
Weight : <500g
OCT scan range : 4 x 4 mm (Horizontal) x 3mm (Depth)
2D image frame rate : ~20 frame/sec
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7. Material & Methods Material & Methods
In this study, we made U87-Luc human glioma cell xenograft mouse models to check the intraoperative usefulness of OCT imaging in glioma surgery. To extend OCT imaging depth, we developed an OCT system with 1300 nm wavelength range. The OCT system enhanced image contrast difference between gliomas and normal brain tissue. Fluorescence imaging and OCT imaging were done and accuracy of the images was analyzed. Moreover, hematoxylin and eosin (H&E) staining was done for each xenograft mouse model to confirm the margin of glioma. Both ex-vivo and in-vivo experiments were performed.
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8. Material & Methods Material & Methods
In this study, we made U87-Luc human glioma cell xenograft mouse models to check the intraoperative usefulness of OCT imaging in glioma surgery. To extend OCT imaging depth, we developed an OCT system with 1300 nm wavelength range. The OCT system enhanced image contrast difference between gliomas and normal brain tissue. Fluorescence imaging and OCT imaging were done and accuracy of the images was analyzed. Moreover, hematoxylin and eosin (H&E) staining was done for each xenograft mouse model to confirm the margin of glioma. Both ex-vivo and in-vivo experiments were performed.
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9. Results Noninvasive OCT imaging of tumor In vivo 3D OCT imaging 1 mm
Margins of the gliomas in xenograft mouse models were compared with both fluorescence images and OCT images. Comparing both images, OCT imaging for glioma was highly accurate. The margin of glioma was confirmed by H&E staining.
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10. Results Comparing maximum area by “ImageJ” : 0.43±0.69㎟ OCT Pathology
Margins of the gliomas in xenograft mouse models were compared with both fluorescence images and OCT images. Comparing both images, OCT imaging for glioma was highly accurate. The margin of glioma was confirmed by H&E staining. 1 2 3 4 5 6 7 8 9 10
OCT
1.03
3.17
2.47
1.11
2.14
2.97
3.20
1.87
1.04
0.88
Pathology
1.79
3.90
3.44
1.72
2.92
3.66
2.89
1.53
2.33
0.00
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11. Conclusion This technology could be a new innovative tool
This feasibility study has demonstrated that intraoperative use of OCT imaging can discriminate clearly between remnant tumor and normal brain.
This technology could be a new innovative tool
for intraoperative imaging in glioma surgery.
Conclusion
This feasibility study has demonstrated that intraoperative use of OCT imaging can discriminate clearly between remnant tumor and normal brain. This technology could be a new innovative tool for intraoperative imaging in glioma surgery
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12. References
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