1. Products > HT-29 Transfection Reagent (Colorectal Adenocarcinoma)
Altogen labs
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > HT-29 Transfection Reagent (Colorectal Adenocarcinoma)
Altogen Biosystems offers the HT-29 Transfection Reagent among a host of 100+ cell line specific In Vitro Transfection Kits.
HT-29 Transfection Reagent is an advanced formulation of a nanoparticle-based liposome formulation, and it is developed to provide high transfection efficiency with HT-29 and other gastric adenocarcinoma cell lines.
This cell line is a good host for studying stomach cancer and human infections related to the stomach.
Other applications include drug discovery, gene expression studies, molecular and cell biology research applications.
Purchase HT-29 Transfection Kit at
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA
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2. High Density HT-29 Cell Line
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > HT-29 Transfection Reagent (Colorectal Adenocarcinoma)
HT-29 Cell Line Description and Applications
The HT-29 cell line originates from the colon tumor cells of a 44-year old woman who suffered from colon adenocarcinoma. The cells were derived in The HT-29 cells are human intestinal epithelial cells and can differentiate into grade I tumors. The cell line is also heterotransplantable. The HT-29 cell line produces the carcinoembryonic antigen (CEA) as well as the secretory component of Immunoglobulin A (IgA), and although the cell line expresses urokinase receptors, it does not express galactose ceramide. The HT-29 cell line has applications for studying cancers as well as studying epithelial differentiation in vitro.
High Density HT-29 Cell Line
Atcc.org
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA
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3. HT-29 Transfection Protocol
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > HT-29 Transfection Reagent (Colorectal Adenocarcinoma)
HT-29 Transfection Protocol
Plate 10, ,000 HT-29 cells per well in 0.5 ml of complete growth medium 12–24 hours prior to transfection
Wash with 1xPBS and add 0.5 ml of fresh growth medium
Prepare transfection complexes by mixing 40 µl of serum free medium, 5.5 µl of transfection reagent, and • 750 ng DNA (or mRNA), or • 30 nM - 50 nM of siRNA (or microRNA) *Referred to a final volume including growth medium
Incubate transfection complexes at RT for minutes
Optional: Add 2 µl of Complex Condenser. This reagent reduces the size of transfection complex, therefore increasing transfection efficiency; however it may increase cell toxicity
Add prepared transfection complexes to 0.5 ml of complete growth medium with HT-29 cells (from step 2)
Incubate cells at 37ºC in a humidified CO2 incubator
Assay for phenotype or target gene expression hours after transfection
Optional: Transfection efficiency can be increased by addition of Transfection Enhancer reagent. Add 2 µl of Transfection Enhancer reagent hours after transfection
If the viability of HT-29 cells being transfected is affected at hours post-transfection, the level of cytotoxicity can be decreased by changing the growth medium and eliminating redundant exposure of cells to transfectant
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA
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4. General Lipoplex-mediated Transfection Mechanism of Action
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > HT-29 Transfection Reagent (Colorectal Adenocarcinoma)
General Lipoplex-mediated Transfection Mechanism of Action
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA
Telephone   Fax   

5. HT-29 Transfection Kit Product Details
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > HT-29 Transfection Reagent (Colorectal Adenocarcinoma)
HT-29 Transfection Kit Product Details
A nanoparticle-based liposome formulation
Optimized easy-to-use transfection protocol provided for transfection of siRNA, DNA, mRNA, and microRNA
Kit includes Transfection Enhancer reagent and recommended transfection protocol
High transfection efficacy in the presence of serum
Expand your RNAi application with a reagent optimized for delivery of both siRNA and plasmid
Reproducible transfection results
Works well for standard reverse transfection and high-throughput applications
Developed and manufactured by Altogen Biosystems (
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA
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6. Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > HT-29 Transfection Reagent (Colorectal Adenocarcinoma)
Data
Figure 1. siRNAs targeting Lamin A/C mRNA or non-silencing control siRNA were transfected into HT-29 cells following the recommended protocol. At 48 hours post-transfection the cells were analyzed by qRT-PCR for Lamin A/C gene expression levels. 18S rRNA levels were used to normalize the Lamin A/C data. Values are normalized to untreated sample. Data are means ± SD (n=4).

7. Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > HT-29 Transfection Reagent (Colorectal Adenocarcinoma)
Data
Figure 2. Protein expression of Lamin A/C in HT-29 cells. DNA plasmid expressing Lamin A/C or siRNA targeting Lamin A/C were transfected into HT-29 cells following Altogen Biosystems transfection protocol. At 72 hours post-transfection the cells were analyzed by Western Blot for protein expression levels (normalized by total protein, 10 µg of total protein loaded per each well). Untreated cells used as a negative control.

8. HT-29 Transfection Kit Benefits
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > HT-29 Transfection Reagent (Colorectal Adenocarcinoma)
HT-29 Transfection Kit Benefits
Pre-optimized transfection protocol for HT-29 cell line
Compatible with DNA, small RNA (siRNA, shRNA, miRNA), mRNA, and small protein complexing
Free of serum and protein of animal origin
Compatible with standard and reverse transfection methods (both protocols provided in the kit manual)
Easy to use HT-29 transfection protocol ensures great performance with expedited experimental timeline
Equally efficient for single or multiple transfections
Can be used for transient transfection and development of stable HT-29 cell lines
Bio-degradable after endocytosis
Used for preclinical research worldwide
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA
Telephone   Fax   