1. Use of computer-assisted sperm analysis and flow cytometry to detect seasonal variations of bovine semen quality 
E. Malama, Y. Zeron, F. Janett, M. Siuda, Z. Roth, H. Bollwein 
Theriogenology 
Volume 87, Pages (January 2017)
DOI: /j.theriogenology
Copyright © 2016 Elsevier Inc. Terms and Conditions

2. Fig. 1
Mean values (±standard deviation) of the percentage of sperm with intact plasma membrane and acrosome (PMAI, %), assessed after 0 and 3 hours of incubation postthaw, in relation to the season of semen collection. Semen samples were collected from five Holstein-Friesian bulls (A–E), during summer (n = 43) and winter (n = 43).
Theriogenology  , 79-90DOI: ( /j.theriogenology )
Copyright © 2016 Elsevier Inc. Terms and Conditions

3. Fig. 2
Mean values (±standard deviation) of the percentage of sperm with high mitochondrial membrane potential (HMMP, %), assessed after 0 and 3 hours of incubation postthaw, in relation to the season of semen collection. Semen samples were collected from five Holstein-Friesian bulls (A–E), during summer (n = 43) and winter (n = 43).
Theriogenology  , 79-90DOI: ( /j.theriogenology )
Copyright © 2016 Elsevier Inc. Terms and Conditions

4. Fig. 3
Mean values (±standard deviation) of the percentage of sperm with low intracellular Ca+2 levels (LOW-Ca+2, %), assessed after 0 and 3 hours of incubation postthaw, in relation to the season of semen collection. Semen samples were collected from five Holstein-Friesian bulls (A–E), during summer (n = 43) and winter (n = 43).
Theriogenology  , 79-90DOI: ( /j.theriogenology )
Copyright © 2016 Elsevier Inc. Terms and Conditions

5. Fig. 4
Mean values (±standard deviation) of the percentage of sperm with high DNA fragmentation index (DFI%, %), assessed after 0 and 3 hours of incubation postthaw, in relation to the season of semen collection. Semen samples were collected from five Holstein-Friesian bulls (A–E), during summer (n = 43) and winter (n = 43).
Theriogenology  , 79-90DOI: ( /j.theriogenology )
Copyright © 2016 Elsevier Inc. Terms and Conditions

6. Fig. 5
Mean values (±standard deviation) of the survival rate of sperm under hypotonic conditions (HYPO-SURV, %), assessed after 0 hours of incubation postthaw, in relation to the season of semen collection. Semen samples were collected from five Holstein-Friesian bulls (A–E), during summer (n = 43) and winter (n = 43).
Theriogenology  , 79-90DOI: ( /j.theriogenology )
Copyright © 2016 Elsevier Inc. Terms and Conditions

7. Fig. 6
Mean values (±standard deviation) of the percentage of sperm with inducible acrosome reaction (IAR, %), assessed after 3 hours of incubation postthaw, in relation to the season of semen collection. Semen samples were collected from five Holstein-Friesian bulls (A–E), during summer (n = 43) and winter (n = 43).
Theriogenology  , 79-90DOI: ( /j.theriogenology )
Copyright © 2016 Elsevier Inc. Terms and Conditions