1. A three-dimensional representation of flow cytometry data
3D-CYTOFLOW
A three-dimensional representation of flow cytometry data

2. Flow Cytometry? Flow Cytometer Analyzer
High Dimensional Data (.fcs files)
Cancer Detection
Ryan

3. Former Solution A set of simple 2D graphs for each pair of properties
Smaller samples
Less accurate
Ryan

4. Our Solution: SANJAY algorithm
Receives a high-dimensional dataset and outputs a 2D/3D representation of it
Reduces the amount of information that a pathologist has to understand
Minimizes the amount of data lost in translation
Ryan

5. Goal
Create a simple, intuitive, and easy to use web portal to allow cancer pathologists to upload flow cytometry data and visualize it in 3D space
Ryan

6. Functionality Two user roles: Admin and Doctor
An admin may add/remove doctors
A doctor may add/remove patients,
upload .fcs files, and view the output of the algorithm
Ryan

7. Registration System
The admin adds the doctor, and the doctor receives an with the token
The doctor uses the token to authorize their account
Now the doctor has access
Luis

8. Technologies
Luis

9. Architecture
Luis

10. Database Schema
Luis

11. Data Flow
Luis

12. VM <-> Web Server
Scheduling with crontab
HTTP Requests with curl
File transfer with Azure Command Line Interface
Manuel

13. VM Scripts Workflow
Manuel

14. Website <-> Web Server
Asynchronous calls with jQuery|AJAX to upload and download data, and to store/retrieve clusters
Three-dimensional output with three.js
Manuel

15. Challenges Migrating initial prototype from MVC 5 to MVC 6
Linux learning curve
Three.js and webGL limitations and documentation
Funding
Manuel