1. Assist. Prof. Mojca Pavlin Head of the Group for nano and biotechnological applications University of Ljubljana, Faculty of electrical engineering/Faculty of Medicine, Slovenia
Expertise in:
nanoparticles development and characterisation: magnetic, gold, liposomes, functionalisation (DLS, zeta potential, nano-chemical lab)
cell labelling and tracking using magnetic nanoparticles, uptake, localisations using live fluorescence imaging , TEM, SEM microscopy, spectrofluorometry, flow cytometry
gene delivery and silencing delivery of small molecules, proteins, DNA and short RNAi into various mammalian, tumour and primary human cells using electroporation, nanoparticles (high-voltage generators)
in vitro cell cultures, viability assays
cell signalling related to cell stress, inflamation, neurodegenaration, cancerogenesis (Western Blot, PCR)
numerical modelling, FEM (Comsol Multiphysics)

2. Expected benefits and activities during participation in BIONECA:
would like to collaborate with groups that use nanoparticles for stem cells labelling
would like to share and collaborate with groups that are interested in studies of potential neurotoxicity of nanoparticles, cell signalling
would like to offer gene transfer/silencing methodology to groups in field of neurology and brain diseases
would like to collaborate with groups with advance in vitro models of brain/neurons
searching partners for new projects applications
Foreseen maximum contribution: WG1 and WG2