1. Day 08
Warm Up- You and your partner will discuss DNA replication vs transcription after watching the 2 videos
DNA Replication
Transcription/Translation Video

2. Title: Translation
Page: 31 EQ: How does Translation lead to the production of protein? Date: 10/21

3. Dealing with this part today!
Central Dogma
Dealing with this part today!
DNA
mRNA
Protein
Translation
Transcription
Nucleus
Ribosome

4. Transcription Review and Translation Intro.

5. Translation:
decoding of an mRNA message into a polypeptide(Protein) chain
occurs on a ribosome (OUTSIDE the nucleus)

6. Translation:
mRNA travels outside of the nucleus and connects to the ribosome
Nucleus
Cytoplasm

7. 2. transfer RNA (tRNA) brings amino acids to the ribosome

8. 3. The ribosome connects the amino acids to form a polypeptide chain (protein)
Protein (Polymer)
Amino Acid (Monomer)

9. How does tRNA “know” what amino acid to bring?

10. Codon GCU CGA Anticodon
Codon: three consecutive mRNA bases
Ex: GCU
Anticodon: three complementary tRNA bases
Codon
GCU
CGA
Anticodon

11. tRNA looks for the codon that pairs with its anticodon
Amino Acid
tRNA looks for the codon that pairs with its anticodon
the ribosome takes the amino acid and attaches it to the polypeptide chain
tRNA
Anticodon
mRNA

12. ORDER MATTERS!
order of DNA bases  order of RNA bases  order of amino acids  what protein is made
tRNA
Amino acids
mRNA

13. How to use the Codon Chart:
Use the left side to find the first letter in the codon
Use the top to find the second letter in the codon
Use the right side to find the third letter of the codon
Go to where ALL three overlap on the chart

14. DNA = GTA
mRNA = CAU

15. Did you notice?: The codon “AUG” can specify methionine or serve as the “start” codon for protein synthesis.

16. How to use the Codon Wheel:
Begin in the middle with the first letter of the codon
Go outward to the second letter in the codon
Go outward again to the third letter in the codon.
DNA = ATC/ATA
mRNA = UAA/UAG

17. What other codons will tell the ribosome to stop putting amino acids together? What DNA sequences will give you these codes?

18. Fill in your mRNA strand from you DNA Fill in your tRNA from your mRNA
Circle a Codon
Use your Codon wheel or Chart to find your amino Acid
DNA
mRNA A G G C G C G U C U C G A U C
tRNA U C C G C G C A G A G C U A G
Arginine
Arginine
Amino Acid
Valine
Serine
Isoleucine
***Remember we use CODONS to read our CODON chart… not the anticodons. Anticodons just bring the correct amino acid.

19. Codon Bingo! Directions For Playing:
If a codon (example: AAG) is called out, you must find the amino acid.
If an amino acid (example: serine) is called out, you must find the codon.
Hint! When finding a codon, there may be more than one answer…you can count all possible answers on your card!