1. New NGS products

2. New products 2016
LinkX EAP
Plans 2017

3. New NGS products 2016 NGSgo-AmpX Whole Gene DQB1 RUO
GenDx-LongTaq
Ion Torrent adapters 4x48 RUO

4. HLA-LinkX HLA Disease Associations with NGS
Compatible with NGSgo workflow for HLA typing
Reduce laboratory workload
Cost effective
B27 alleles strongly predispose for Ankylosing Spondylitis
In ~90% of European patients

5. HLA-LinkX B DQB1 DQA1 Disease Ankylosing spondylitis Celiac disease
Consensus typing
Ankylosing spondylitis
B27
Celiac disease
DQ2.5
DQ8
DQ2.2
DQ7.5
Narcolepsy
DQB1*06:02
Behcet’s disease
HLA-B*51
HLA-B*52
Abacavir hypersensitivity
HLA-B*57:01 1 4 3 2 7 6 5
0.9 kB
1.3 kB B
DQA1
0.4 kB (Ex3)
0.6 kB (Ex2)
DQB1
B27 alleles strongly predispose for Ankylosing Spondylitis
In ~90% of European patients

6. In the upper left there’s a filter option for the gene of interest
Please find here an impression of the prototype version of NGSengine that is currently under development for HLALinkX.
In the upper left there’s a filter option for the gene of interest
Samples analyzed are listed and results are marked positive, negative, or undecisive
Quality scores and the level of confidence are given
as well as the HLA groups assigned to the sample.
In case of unclear results, additional information can be obtained by clicking on details. (KLIK)
NGSgo-HLALinkX Prototype

7. The qualifier shows for instance: -Coverage measures (KLIK)
This view allows further insight into the quality scores, including the acceptance criteria, the measured value and whether the acceptance criteria is met.
The qualifier shows for instance:
-Coverage measures (KLIK)
-Heterozygocity index for balanced allele fraction and (KLIK)
-the number of crucial positions passed
These quality scores can help the user to decide whether a sample can be approved yes or no.
NGSgo-HLALinkX Prototype

8. NGSgo®-HLALinkX Features
NGS-based HLA disease association assay
Single tube solution
Compatible with NGSgo® HLA typing workflow
Streamlined workload by
combining HLALinkX and AmpX amplicons in a single run
Comprehensive results with NGSengine
Compatible with Illumina NGS Instruments
Taken together,
We developed a robust NGS-based HLA disease association assay that amplifies the essential regions of HLA-B, DQA1, and DQB1 in a single tube
The NGSgo-HLALinkX assay is fully compatible with the standardized NGSgo® workflow for high resolution HLA typing
Your laboratory workload can be streamlined by combining HLALinkX and AmpX amplicons in a single run.
Comprehensive results are obtained with NGSengine
NGSgo-HLALinkX assay is compatible with all Illumina NGS instruments
NGSgo-HLALinkX Early Access Program

9. NGSgo®-HLALinkX EAP Goals: Q1: HLA-B27 Q4: B27, B*57:01, CD
Feedback from potential customers
Performance
How many tests
Pricing
Q1: HLA-B27
Q4: B27, B*57:01, CD
If your laboratory is performing disease association or pharmacogenetics tests and is using our NGSgo workflow on an Illumina Platform,
you can be the first to use our upcoming NGSgo-HLALinkX.
This will give you the opportunity to make suggestions that will suit your specific needs, for example additional loci or diseases to add to the workflow.
Please contact us in case you are interested
NGSgo-HLALinkX Early Access Program

10. Plans 2017

11. General goals Develop innovative products Partnering R&D
Abstracts, publications

12. NGS Projects NGSgo workflow upgrade Automation bead merge
Beckman, Hamilton, Tecan, Perkin Elmer
IndX plates

13. NGS Projects Amplifications Whole gene Class II Whole gene HLA-G
DRB1, DRB345, DPB1, DPA1
Whole gene HLA-G
Other genes: KIR, MICA, MICB

14. NGS Projects
HLA typing on Oxford Nanopore
Chimerism / NGS

15. NGSengine New allele submitter Autostart analyse Database management
Missing data
Quality metrics
Oxford Nanopore Technologies