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Presented By: Sarah A. Gregory

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1 Light Exposure Increases Dissolved Organic Carbon Uptake in Wyoming Rivers
Presented By: Sarah A. Gregory Co-Authors: Erin R. Hotchkiss & Robert O. Hall, University of Wyoming

2 Acknowledgements Special Thanks to: NSF REU Program UW EPSCoR Program
ENR Haub Research and Creative Activities Grant Honors Program NSF Big Rivers Project Summer 2010 In Collaboration with Utah State University, University of Notre Dame, and Cary Institute of Ecosystem Studies

3 Inland Waters Assumed to be Passive Carbon Transporters

4 Past Assumption: Inland Waters Are Pipes

5 Inland Waters Process More than ½ of Carbon From Land
Initial Model New Model

6 Dissolved Organic Carbon Represents a Majority of Total Organic Carbon In Inland Waters
Known as DOC <0.45μm DOC enters inland waters from: External Loading = Terrestrial Internal Processes = Algal

7 Available DOC can be consumed by bacteria
Availability based on structure of DOC Highly available is readily consumed by microbes DOC source is linked to the availability of DOC Due to structure of DOC from source

8 Light Exposure Increases DOC Availability
UV changes molecular structure High molecular weight is less available Low Molecular Weight High Molecular Weight

9 Research Questions What is the effect of light exposure on uptake?
What is the interaction between uptake and spectral absorbance (indicator of molecular weight)?

10 We Added Leaves to Water to Leach Terrestrial DOC
Soil Rock Scrubbings

11 Light and Dark Treatments to Quantify the Changes of DOC Availability

12 Biological Assays are Used to Calculate Bacterial Consumption of DOC
Filtered Water + Bacterial Inoculum Change in DOC from start to end is the total DOC consumed by bacteria Bacterial Consumption=Uptake=K (d-1)=(-ln(docend-docstart)) / time

13 Light Exposure Increases Uptake -Laramie River

14 Light Exposure Increases Uptake -Snake River
2 Way ANOVA Statistically Significant

15 Biofilm River Water Leaves
Changes in Molecular Structure Due to Light is Source Dependent Biofilm River Water K (d-1) Light Dark Leaves Soil Low (250): High MW (365)

16 Summary Light exposure increased bacterial uptake of DOC
Changes in DOC structure after light exposure depends on source

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