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Pioneer identification of fake tiger claws using morphometric and DNA-based analysis in wildlife forensics in India  Vipin, Vinita Sharma, Chandra Prakash.

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Presentation on theme: "Pioneer identification of fake tiger claws using morphometric and DNA-based analysis in wildlife forensics in India  Vipin, Vinita Sharma, Chandra Prakash."— Presentation transcript:

1 Pioneer identification of fake tiger claws using morphometric and DNA-based analysis in wildlife forensics in India  Vipin, Vinita Sharma, Chandra Prakash Sharma, Ved Prakash Kumar, Surendra Prakash Goyal  Forensic Science International  Volume 266, Pages (September 2016) DOI: /j.forsciint Copyright © 2016 Elsevier Ireland Ltd Terms and Conditions

2 Fig. 1 Photographs of seized claws (n=18).
Forensic Science International  , DOI: ( /j.forsciint ) Copyright © 2016 Elsevier Ireland Ltd Terms and Conditions

3 Fig. 2 Points used for claw measurements.
Forensic Science International  , DOI: ( /j.forsciint ) Copyright © 2016 Elsevier Ireland Ltd Terms and Conditions

4 Fig. 3 Keratinous claw sheath of tiger claw with a uniform curved line seen (A); grinding marks on surface of seized claw (B) and air bubbles and white thread-like structure observed in four seized claws (C). Forensic Science International  , DOI: ( /j.forsciint ) Copyright © 2016 Elsevier Ireland Ltd Terms and Conditions

5 Fig. 4 X-rays showing varying keratin density in tiger (A) and leopard (B) claws and homogeneous density in the seized claws (C). Forensic Science International  , DOI: ( /j.forsciint ) Copyright © 2016 Elsevier Ireland Ltd Terms and Conditions

6 Fig. 5 Discrimination of tiger, leopard and seized claws on the basis of canonical discriminant functions. Forensic Science International  , DOI: ( /j.forsciint ) Copyright © 2016 Elsevier Ireland Ltd Terms and Conditions

7 Fig. 6 Visualization of genomic DNA and PCR products on 0.8% and 2.0% agarose gels, respectively. Lane 1 shows the genomic DNA of one of the four claws. Lanes 4 and 7 show the PCR-amplified products of the cyt b and 16S rRNA genes, respectively. Lanes 2, 5 and 8 are negative controls. Lanes 3, 6 and 9 show a molecular weight marker of 100bp. Forensic Science International  , DOI: ( /j.forsciint ) Copyright © 2016 Elsevier Ireland Ltd Terms and Conditions

8 Fig. 7 BLAST search using cyt b (A) and 16S rRNA (B) on the NCBI, USA database. Forensic Science International  , DOI: ( /j.forsciint ) Copyright © 2016 Elsevier Ireland Ltd Terms and Conditions

9 Fig. 8 NJ trees of cyt b (A) and 16S rRNA (B). The numerical values at the nodes represent the bootstrap values above 50% with 1000 replicates. Forensic Science International  , DOI: ( /j.forsciint ) Copyright © 2016 Elsevier Ireland Ltd Terms and Conditions

10 Fig. 9 Suggested hierarchical process for differentiation of tiger/leopard claws from fake claws. Forensic Science International  , DOI: ( /j.forsciint ) Copyright © 2016 Elsevier Ireland Ltd Terms and Conditions


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