1. Chemical Examination of Urine
Lesson 5-4
Chemical Examination of Urine

2. Urine Chemical Tests Reagent strips CLIA-waived Rapid results
Multiple tests on one strip
CLIA-waived
Rapid results

3. Urine Chemical Tests
Color end-point
Timed

4. Principles of Reagent Strip Tests
Glucose
Bilirubin
Ketones

5. Principles of Reagent Strip Tests
Blood pH
Protein
Urobilinogen

6. Principles of Reagent Strip Tests
Nitrite
Leukocyte esterase
Specific gravity
Reference values
See Table 5-11

7. Special Reagent Strips
Glucose
Ketones
Microalbumin
hCG

8. Copper Reduction and Confirmatory Tests
Reducing sugars
Protein precipitation
Bilirubin
Ketones

9. Reagent Strip Procedure
Safety precautions
Quality assessment
Specimen
Room temperature
Well-mixed
Reagent strips
Urine controls

10. Reagent Strip Procedure
Manual method

11. Reagent Strip Readers
Varying capacities

12. Using the Clinitek Status

13. Copper Reduction Test Clinitest Detects reducing sugars
Use for sugars other than glucose
Galactosuria
Caustic reagent

14. Copper Reduction Test

15. Confirmatory Tests Protein Acid precipitation
Dilute acetic acid
Dilute sulfosalicylic acid
Albumins and globulins react
Amount of precipitation proportional to
protein concentration
Grade reaction: trace, 1+ to 4+

16. Confirmatory Tests Ketones – Acetest Bilirubin – Ictotest
This project was funded at $3,000,000 (100% of its total cost) from a grant awarded under the Trade Adjustment Assistance Community College and Career Training Grants, as implemented by the U.S. Department of Labor’s Employment and Training Administration. Rogue Community College is an equal opportunity employer/program. Auxiliary aids and services, alternate form and language services are available to individuals with disabilities and limited English proficiency free of cost upon request.
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