1. Functional group: ADME
NEW from Shiseido
CAPCELL PAK INERT ADME
CAPCELL PAK INERT ADME S3 2 4 6 8 10 12
[min]
EXCELLENT Peak Shape !
Diphacinone M.W. :
Conventional C18 column S3
【HPLC Conditions】 Column size : 2.0 mm i.d. x 50 mm Mobile phase : 10 mmol/L HCOONH4 / CH3CN = 70 / 30 Flow rate : 0.2 mL/min Temperature : 40 ℃ Detection : UV 285 nm
Inj. vol. : 1 mL
Sample dissolved in : 1 mg/mL in CH3CN, diluted with 20% CH3CN at 10 mg/mL. 2 4 6 8 10 12
[min]
CAPCELL PAK INERT ADME S3
AMP
Large Retention Times for Polar Compounds!
k = 1.71
[min] 1 2 3 4 5 6
ADP
k = 0.56
ATP
k = 0.20
ATP M.W. :
【HPLC Conditions】 Column size : 2.0 mm i.d. x 150 mm Mobile phase : 10 mmol/L HCOONH4 / CH3CN = 99 / 1
Flow rate : 0.2 mL/min Temperature : 40 ℃ Detection : UV 260 nm Inj. vol. : 1 mL
Sample dissolved in : H2O (10 mg/mL each.)
k = 1.05
k = 0.31
Competitor’s metal-free ODS column 3 mm
k = 0.12
Realized by:
Analyte
Strong interaction
Inert column
Functional group: ADME
Inner surface : PEEK
Filter : PEEK

2. Characteristics Severe peak tailings or no peaks !?
Adsorbed to the column ?
Metal coordination suspected ?
Hard to retain hydrophilic compounds ?
Cephem antibiotics　～Ceftriaxon～
Ceftriaxon
M.W. :
Ceftriaxon
CAPCELL PAK C18 MGIII-H S3
[min]
1.0
2.0
3.0
4.0
5.0
CAPCELL PAK INERT ADME S3
CAPCELL PAK ADME S3 １ 3 4 5 6 7
5,6
CAPCELL PAK INERT ADME S3
0.0
1.0
2.0
3.0
4.0
[min]
S/N = 20.5
0.0
1.0
2.0
3.0
4.0
[min]
S/N = 10.1
CAPCELL PAK ADME S3
MRM　555->396
Nucleotide
【HPLC Conditions】
Column Size : 2.0 or 2.1 mm i.d. × 50 mm　
Mobile phase : A) 0.1 vol% HCOOH, B) 0.1 vol% HCOOH, CH3CN
PDA ; B 15% -> 95% (3 min) MS ; B 15% -> 95% (2 min)
Flow rate : 0.5 mL/min
Temperature : 40˚C
Detection : PDA 260 nm, MS ESI positive
Inj.vol. : 1 mL
Sample : PDA 20 mg/mL each. MS 200 ng/mL 1 2 3 4 5 6
Conventional C18 column S3
1. Cephapirin
2. Ceftriaxone
3. Cefazolin
4. Cephaperazone
5. Cephalothin
6. Sefaca pen Pivoxil
7. Cefditoren pivoxil 1 2 3 4 5 6
[min]
346->79　(AMP)
426->79　(ADP)
506->159 (ATP)
N.D.
ATP
N.D.
Characteristics
【HPLC Conditions】 Column size : 2.0 mm i.d. x 150 mm Mobile phase : 10 mmol/L HCOONH4 / CH3OH = 98 / 2
Flow rate : 0.2 mL/min Temperature : 40 ℃ Detection : MS ESI negative
Inj. vol. : 2 mL
Sample dissolved in : H2O (150 ng/mL each.)
CAPCELL PAK INERT ADME S3
*　All date acquired by NANOSPACE 2 HPLC system (Inert specification).
P/N
I.D (mm)
Length (mm)
79001 2 50
79002
100
79003
150