1.
최장현

2. Gastric Cancer 조기위암의 육안분류 진행위암의 보우만분류

3. SOX9(Sry-related HMG box9)
a. Normal epithelium
b. Gastric carcinoma

4. SOX9(Sry-related HMG box9)
w: weeks postinjection

5. Figure 1. SOX9 is upregulated in human gastric cancer
Gastric Cancer Subtypes.
CIN: Chromosomal instability
EBV: Epstein-Barr virus
GSI: Genomically stable
MSI: Microsatellite instability
TCGA(The Cancer Genome Atlas)
ACRG(Asian Cancer Research Group)
MSS: Microsatellite stability
EMT: Epithelial-to-
mesenchymal transition
OS: Overall Survival
DFS: Disease-Free Survival

6. Figure 2. Tumor suppressor phenotype induced by Sox9 and β-catenin knockdown
PARP1: Poly [ADP-rebose] polymerase1
role in repair of single-stranded DNA breaks
Caspase-3: Cysteine-ASPartic acid protease-3
role in the execution-phase of cell apoptosis
Cip: CDK-interacting protein(cip1=p21, inhibition of cell cycle)
BMI-1: target of SOX9 and regulator of p21cip, inhibition of
senescence
SA-β-Gal: senescence associated beta-galactosidase
SYBR
Primer: 0.2μmol/L
cDNA: 20ng/well

7. Figure 2. Tumor suppressor phenotype induced by Sox9 and β-catenin knockdown

8. Figure 3. SOX9 is required for gCSC maintenance and tumor initiation capacity
CD44, OCT4, BMI-1, CD133: Cancer Stem Cell Marker
Tumor initiating control cells
pLKO 1/14.299
sh1 1/
sh2 1/
Ki-67: cellular marker for proliferation

9. Figure 4. SOX9-β-catenin signaling axis in response to H
Figure 4. SOX9-β-catenin signaling axis in response to H. pylori infection

10. Figure 4. SOX9-β-catenin signaling axis in response to H
Figure 4. SOX9-β-catenin signaling axis in response to H. pylori infection
695, 742 strain: cagA+/vacAs1m1
904, 803 strain: cagA-/vacAs2m2
cagA: Cytotoxin-associated gene A
vacA: Vacuolating cytotoxin A

11. Figure 4. SOX9-β-catenin signaling axis in response to H
Figure 4. SOX9-β-catenin signaling axis in response to H. pylori infection

12. Figure 5. H. pylori activity is mediated by SOX9-β-catenin signaling
HP+: H. pylori-infected
HP- H. pylori- noninfected

13. Figure 5. H. pylori activity is mediated by SOX9-β-catenin signaling
IL4: Interleukin 4,
inducing stimulation of activated B-cell and T-cell proliferation
IL6: Interleukin 6,
mediator of fever and of the acute phase response
IL1β: Interleukin 1β, leukocytic pyrogen
TNFα: Tumor Necrosis Factor α

14. Figure 6. SOX9 modulates chemotherapy response
Cisplatin: 10 μmol/L
Cisplatin: 1 μmol/L
Cisplatin: chemotherapy medication used to treat a number of cancers

15. Figure 6. SOX9 modulates chemotherapy response
SOX9-overexpressing MKN45
MKN45

16. Summary SOX9 expression is consistently elevated in gastric cancer
Tumor cells depend on SOX9 to survive and proliferate
SOX9 acts downstream β-catenin in gastric cancer
SOX9 elevation acts with canonical wnt signaling to drive gastric cancer progression