1. Naturally Programmed Cell Death, Apoptosis, and Cancer
Folder Title: Apoptose
or ...
December 1, 2004
TtlApop

2. What happened to your webbed feet?
You guys are dethpicable.
DaffyDuc

3. God and Michelangelo Creation by Subtraction Evolution:
Selective Reproduction
Selective Death
Subtract

4. Selective Cell Death in Normal Development and Normal Organismic Functioning
Developmental Remodelling
Tadpole to Frog
Human Fetal Remodelling
Immunological Self-Tolerance
Normal Physiology
Post-Lactation Breast Involution
Menstruation
Normal Immune T-Cell Killing
NormApop

6. Necrosis vs Apoptosis Necrosis Apoptosis Clumped Chromatin
Swelling of Organelles
Cell Distension
Membrane Disruption
Inflammation
Apoptosis
Condensed Nuclear Fragments
Shrunken Cytoplasm
Membrane Blebs (Apoptotic Bodies)
Phagocytosis without Inflammation
Kill&Die

7. Apoptotic Cell. Ruddon, 3rd Ed. Fig. 10-7
ApoCell

8. from Scientific American, December, 1996
Membrane Blebs
on a Cell
Undergoing
Apoptosis
from Scientific American,
December, 1996
ApoBlebs

9. DNA Lysis into "DNA Ladder". DNA from Apoptotic Cell.
From Ruddon, 3rd Ed.,
Figure 10-8
ApoDNA

10. Genetic Control of "Genetically Programmed Cell Death"
Cellular Location of Protein Product
Mitochondrial Membrane
Nuclear Envelope
Endoplasmic Reticulum
Nucleus
Cell Membrane
Apoptosis Gene
Effect on Apoptosis
Blocks
Stimulates
Wild-type Stimulates
Mutant Blocks
bcl-2
myc
p53
p53*
APO-1/
FAS
ApoGenes

11. Exogenous Genetic Suppressors of Apoptosis
Adenovirus E1b Oncoprotein
Ras
v-abl
compox crm a gene product
ExoApop1

12. Exogenous Inducers of Apoptosis
Cytotoxic T-Lymphocyte Attack on Target Cells
Loss of Growth Factors
Loss of Responses from Neighboring Cells
DNA-Directed Anti-Neoplastic Agents
ExoApop2

13. More Exogenous Inducers of Apoptosis (from Ruddon, 3rd Ed., p. 397)
Differentiation Signals
Removal of Growth Factors or Required Hormones
Exposure to Transforming Growth Factor B (TGF-B)
Tumor Necrosis Factor (TNF)
Glucocorticoids (Immune-modulating Corticosteroids)
DNA-damaging Antineoplastic Agents
Environmental Toxins
Agents Stimulating Ca Influx (Calcium Ionophores)
Cytoskeletal-directed Microtubule Disruptors
Microfilament-directed Cytochalasin B Inhibits formation of Apoptotic Bodies, but not apoptosis itself
ExoApop3

14. Normal T-Cell Activation by
Antigen-Presenting Cells. (from Sci. Am., Dec, 1996)
TCellAct

15. Activated T-Cell Down-regulation via FAS Cell-Surface
Protein and Ligand Binding to FAS. (from Sci. Am., Dec., 1996)
TCelDown