1. KINETICS OF BIOMARKER DEVELOPMENT
BIOSCICON, INC Point-of-Care Technology for Global Health NIH Conference January 2014
A MODEL INSTRUMENT TO MEASURE EFFICACY OF SMALL MOLECULES TO MODIFY METABOLIC KINETICS IN SINGLE CELLS OBTAINED FROM TUMOR TISSUES
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KINETICS OF BIOMARKER DEVELOPMENT

2. FIGHTING CANCER NEEDS INNOVATIONS
CANCER IS DESCRIBED AS UNSTOPABLE GROWTH OF ABNORMAL CELLS WHICH DISTROY NORMAL CELLS AND TISSUE AT POINTS OF ORIGIN, SPREAD AND METASTASES.
CANCER IS A FATAL DISEASE KNOWN FROM THE BEGINNING OF THE HISTORY: ALL ATTMEPTS TO CURE THIS DISEASE, WHEN ADVANCED, HAVE FAILED; CONSEQUENTLY, REPROGRAMMING CANCER GROWTH, IF POSSIBLE, IS THE NEW TARGET OF THE OLD FIGHT AGAINST CANCER.
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KINETICS OF BIOMARKER DEVELOPMENT

3. CHEMOTHERAPY IS NOT PERFECT
CHEMOTHERAPY IS DESIGNED TO KILL CANCER CELLS WILE PRESERVING NORMAL TISSUES.
CHEMOTHERAPY HAS CYTOCIDAL (OXYGEN RADICALS, ANTIBODIES) AND CYTOSTATIC (ANTI MITOTIC) EFFECTS; NORMAL CELLS CANNOT BE COMPLETELY PROTECTED.
BY THEORY, CHEMOTHERAPY AFFECTS A PERCENT OF CANCER CELLS AND CANNOT ELIMINATE THE LAST CELL WHICH CAN GENERATE CANCER REGROWTH.
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KINETICS OF BIOMARKER DEVELOPMENT

4. KINETICS OF BIOMARKER DEVELOPMENT
WHY REPROGRAMMING?
NORMAL CELLS ARE PROGRAMMED TO GROW, DIFFERENTIATE, MATURE AND DIE IN A CONTINUUM OF THEIR LIFE, WICH HAS SUPPORTIVE CONTROL BY THE NORMAL BODY LOGISTICS.
CANCER CELLS DO NOT ABIDE TO BODY CONTROLS AND HAVE ABILITY TO ESCAPE ATTACKS FROM THE IMMUNOSURVEILLANCE AND BODY PROTECTIVE CELLS.
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KINETICS OF BIOMARKER DEVELOPMENT

5. KINETICS OF BIOMARKER DEVELOPMENT
IDEA!
CHANGING METABOLIC PATHWAYS IN ABNORMAL CELLS COULD CAUSE:
PREMATURE APPOPTOSIS (DYING) OR
ELIMINATING FACTORS BLOCKING THE RESPONSE TO NORMAL GROWTH CONTROL MECHANISMS, AND
LETING THEM TO CONTROL FURTHER FAITH OF MODIFIED ABNORMAL CELLS
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KINETICS OF BIOMARKER DEVELOPMENT

6. IN VITRO MODEL TO TEST CELL SENSITIVITY TO DRUGS
A COMBINATION OF AN OPEN WINDOWS CELL CULTURE CHAMBER, A SYSTEM FOR CONTINUOUS MONITORING/RECORDING OF INTRACELLULAR CHEMICAL REACTION, SPECIMEN SMEARED ON A GLASS WALL OF THE CHAMBER, CHROMOGENIC SUBSTRATE, A LIGHT MICROSCOPE WITH OR WITHOUT PHOTOMETER OR CAMERA TO MEASURE THE ABSORBANCE, IS MAKING THE MEASUREMENTS POSSIBLE.
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KINETICS OF BIOMARKER DEVELOPMENT

7. STRUCTURE OF THE MODEL TO TEST CELL SENSITIVITY
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KINETICS OF BIOMARKER DEVELOPMENT

8. SINGLE CELL METABOLIC ANALYSIS
Nanoparticles buildup indicating the presence, location, size and power of bioactive molecules inside single cells.
Monitoring of this reaction, or its recording as time lapse still images or a video, could be a powerful quantitative method for testing cell sensitivity to drugs and toxins and new drugs development.
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KINETICS OF BIOMARKER DEVELOPMENT

9. KINETICS OF BIOMARKER DEVELOPMENT
FUNCTION OF THE MODEL
THE FOLLOWING VIDEO WILL PRESENT HOW THIS MODEL WORKS AND COULD BE USED IN RESEARCH
A NORMAL BLOOD IS SMEARED INSIDE THE INCUBATION CHAMBER.
A NORMAL PML IS FOCUSED IN THE CENTER OF A MICROSCOPIC FIELD
MYELOPEROXIDASE REACTION IS INITIATED BY INTRODICING APPROPRIATE SUBSTRATS
KINETICS OF THE FINAL REACTION PRODUCT DEVELOPMENT IS OBSERVED, RECORDED AND MEASURED
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KINETICS OF BIOMARKER DEVELOPMENT

10. KINETICS OF BIOMARKER DEVELOPMENT
VIDEO
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KINETICS OF BIOMARKER DEVELOPMENT

11. KINETICS OF CATALYSIS MEASURED BY PRODUCT DEVELOPMENT
S, E
S + E
K1 > K2
SEP
K3 > K4
E + P
P*P
K5 > K6
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KINETICS OF BIOMARKER DEVELOPMENT

12. KINETICS OF BIOMARKER DEVELOPMENT
MANAGEMENT
INTRODUCING INHIBITORS AND ACTIVATORS INTO THIS MODEL SYSTEM, IT WAS POSSIBLE TO MODIFY THE REACTION AND TO MEASURE THE EFFECT OF THE NEW MOLECULES TO THE KINETICS OF INTRACELLULAR METABOLISM
CONFIRMING THE FEASIBILITY OF THE MODEL TO ENABLE THE MONITORING AND MEASURING THIS EFFECT WAS A SUFFICIENT EVIDENCE TO WARRANT PATENTING THE MODEL FOR THE APPLICATION IN CANCER REPROGAMMING RESEARCH
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KINETICS OF BIOMARKER DEVELOPMENT

13. KINETICS OF BIOMARKER DEVELOPMENT
TESTING NEW MOLECULES
TARGETS OF MEDICAL INTEREST
MOLECULAR FUNCTIONS TO BE TARGETED
BODY
SYSTEM
ORGAN
TISSUE
CELL
ORGANELLE
DNK
RNA
ACTIVE PROTEIN
K1/K2
K3/K4
K5/K6
INHIBITORS
ACTIVATORS
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KINETICS OF BIOMARKER DEVELOPMENT

14. CERVICAL CANCER CHALLENGE TO CONTROL
CAP + HIV MARKERS POSITIVE CERVICAL CANCER CELLS
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KINETICS OF BIOMARKER DEVELOPMENT

15. KINETICS OF BIOMARKER DEVELOPMENT
CONTACT
Dr. NENAD MARKOVIC
BIOSCICON, INC.
Tel
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KINETICS OF BIOMARKER DEVELOPMENT