1. Practical Pharmacology
Lab.6
3rd-year class
Practical Pharmacology

2. effect of Atropine on man

3. Procedure:-
1.Student are divided into 4 groups. 2.One student of each group acts as a volunteer. 3.The following observations are recorded 5 minutes interval prior to injection of Atropine. a. Subjective symptoms. b. Salivation. c. Pulse rate. d. Size of pupil. e. Reaction to light. f. Accommodation

4. N.B:- to calculate the volume of saliva, we collect saliva for 3 minutes after sucking a drop of ascorbic acid(vitamin c).
4. Injection of Atropine solution 1ml(0.6 mg) is given subcutaneously to each volunteer.
5. The same observations are made after injection, at 10 minutes interval for 40 minutes.

5. 6. Make a table for the obtained data as follows:- Before injection after injection

6. Parameter Subjective Salivation Pulse rate Size of pupil
40 min
30 min
20 min
10 min
5 min
Parameter
Subjective
Salivation
Pulse rate
Size of pupil
Reaction to light
Accommodation