1. Dylan
Mitchell
Pineapple Express
Dannielle
Alex

2. Effects of Acids Strength on Soy Bean Seeds

3. Question
What is the optimal pH for the germination of soy bean (fabaceae) seeds?

5. If the water is less acidic then more seeds will germinate.
Hypothesis
If the water is less acidic then more seeds will germinate.

6. Materials 3 Beakers pH paper Acetic acid Distilled water
12 petri dishes
120 soy bean seeds (Fabaceae)
White tape
2 graduated cylinders
3 stirring rods
Gloves
Aprons
Goggles

7. Procedure Part 1 Part One Procedure:
Gather- Beakers, pH papers, acetic acid, distilled water, stirring rod
Mix distilled water and acetic acid*, then test pH and record
Repeat until desired pH of 4.5, 5.5, and 6.5 are acquired
Save desired mixture for part 2
*Distilled water = 6.5
*Acetic Acid = 4.5
*24 mL distilled water and 0.03g of baking soda = 7
*24 mL of distilled water + 12 mL of acetic acid g of baking soda = 5.5

8. Procedure Part 2 Gather all materials on list
Recreate the acids from part 1
Label each petri dish
Put 10 soybean seeds in each dish
Add 8mL of distilled water to the three petri dishes 4A,4B,4C
Add 8mL of 4.5 solutions to the three petri dishes labeled 1A,1B, 1C
Add 8mL of 5.5 solutions to the three petri dishes 2A, 2B, 2C
Add 8mL of 6.5 solutions to the three petri dishes 3A, 3B 3C
Water as needed.
Put lids on petri dish, sit and record for 7 days

9. # of Seeds Germinated Per Day
Quantitative Data PH
# of Seeds Germinated Per Day
Total Average
Day 1
Day 2
Day 3
Day 4
Day 5
Day 6
Day 7
(4.5) 1a
(4.5) 1b
(4.5) 1c
(5.5) 2a
(5.5) 2b
(5.5) 2c
(6.5) 3a 1 7 8
(6.5) 3b 9
(6.5) 3c
(7) 4a 6
(7) 4b 10
(7) 4c

11. Average Number of Seeds Germinated Per Day
Averages Results pH
Average Number of Seeds Germinated Per Day
Day 1
Day 2
Day 3
Day 4
Day 5
Day 6
Day 7
4.5
5.5
6.5 8 7 9

13. Qualitative Data Smelled terrible throughout the entire lab.
Looked kind of mushy.
Tan color for the seeds that did not germinate,
Pale yellow color with a dark brown patch for the seeds that germinated.
Acid was a milky white color at the end of the lab.
The hypocotyl was a white color.

14. Pictures!! 

15. Conclusion
Based on our data, our hypothesis was supported. More seeds were germinated when the water was less acidic.

16. Problems
The pH paper was expired and did not give us an accurate reading of the 5.5 pH solution. We concluded it was actually more acidic thus not germinating.
In the petri dishes with 7 pH, the seeds started to, what we assumed, decay.
Some acid spillage while counting the number of seeds germinated.

17. Questions
If we had pH paper that hadn’t expired and had we gotten the correct 5.5 pH, how would the seeds have germinated for that group.
How basic (pH) could we have gone until the seeds would not germinate?
How much more accurate would our pH’s have been had we used an electronic pH reader rather than pH paper?
Does the acidity effect different types of seeds differently?
How would the seeds have been effected if we left the seeds in the light rather than in the drawer?