1. Volume 21, Issue 11, Pages 1486-1496 (November 2014)
Inhibition of AMP Deaminase Activity Does Not Improve Glucose Control in Rodent Models of Insulin Resistance or Diabetes 
Therese Admyre, Lena Amrot-Fors, Maria Andersson, Martin Bauer, Mikael Bjursell, Tomas Drmota, Stefan Hallén, Judith Hartleib- Geschwindner, Bo Lindmark, Jianming Liu, Lars Löfgren, Mattias Rohman, Nidhal Selmi, Kristina Wallenius 
Chemistry & Biology 
Volume 21, Issue 11, Pages (November 2014)
DOI: /j.chembiol
Copyright © 2014 Elsevier Ltd Terms and Conditions

2. Figure 1
High-Throughput Screen and Hit Confirmation for the Identification of AMPD1 Inhibitors
(A) Schematic showing the AMPD assay. α-KG, α-ketoglutarate.
(B) Z’ factors of each run through the HTS.
(C) The correlation between the negative logarithm of the IC50 (pIC50) from the HTS assay and the counterscreen. The x axis is the counterscreen, and the y axis is the HTS assay.
(D) Correlation between three-point and seven-point concentration responses. The x axis shows seven-point data, and the y axis shows three-point data.
Chemistry & Biology  , DOI: ( /j.chembiol )
Copyright © 2014 Elsevier Ltd Terms and Conditions

3. Figure 2 Properties and Optimization of AMPD Inhibitors
(A) The structure-activity relationship for series 106.
(B) Structures for compounds 1, 2, 3, and 4.
(C) Direct comparison of the inhibitory action of compound 2 (squares) and compound 3 (circles) in ob/ob muscle homogenates (MH, open symbols, n = 3) with recombinant murine AMPD1 (filled symbols, n = 2, upper panel) as well as rat liver extracts (LH, open symbols, n = 3) and recombinant AMPD2 (filled symbols, n = 6, lower panel). The values are mean ± SD.
Chemistry & Biology  , DOI: ( /j.chembiol )
Copyright © 2014 Elsevier Ltd Terms and Conditions

4. Figure 3 Pharmacological Inhibition of AMPD in Primary Hepatocytes
(A) Fructose induced ATP depletion in primary rat hepatocytes. Freshly prepared rat hepatocytes kept in suspension were ATP-depleted by a 5 min incubation with fructose (2.8 mM) that induced IMP formation.
(B) IMP levels in ATP-depleted hepatocytes treated with AMPD inhibitors. cpd, compound.
(C) Effects on AMP levels in hepatocytes treated with AMPD inhibitors.
The values are mean ± SD. #, below the detection limit of the system; ∗p < 0.03 compared with the DMSO controls in a two-tailed Student’s t test.
Chemistry & Biology  , DOI: ( /j.chembiol )
Copyright © 2014 Elsevier Ltd Terms and Conditions

5. Figure 4
Metabolic effects because of deletion of the Ampd1 gene in mice.
(A) Body weight gain from 4–14 weeks of age in standard chow-fed (SCD) wild-type mice (n = 8, filled circles), Ampd1 KO mice (n = 8, filled circles), HFD-fed wild-type mice (n = 8, filled squares), and Ampd1 KO mice (n = 8, open squares). Statistical analysis was performed using repeated Student’s t test.
(B and C) Oral glucose tolerance test in chow-fed wild-type mice (n = 7, filled circles), Ampd1 KO mice (n = 7, open circles), HFD-fed wild-type mice (n = 7, filled squares), and Ampd1 KO mice (n = 7, open squares). The results are presented as mean ± SEM. Statistical analysis of AUC values was performed using Student’s t test.
Chemistry & Biology  , DOI: ( /j.chembiol )
Copyright © 2014 Elsevier Ltd Terms and Conditions

6. Chemistry & Biology 2014 21, 1486-1496DOI: (10. 1016/j. chembiol. 2014
Copyright © 2014 Elsevier Ltd Terms and Conditions