1. Screening for bioactive chemicals at the CMBA facility
2008
Screening for bioactive chemicals at the CMBA facility
BIG – BGE - CEA-Grenoble
Scientific Responsible : Marie-Odile Fauvarque
Operating Staff: Caroline Barette (HTS)
Emmanuelle Soleilhac (HCS)
Research tools
Therapeutic drugs
Dynamic: temporal control
Reversible : quick inhibition or activation
Dose-dependent
Target identification: new actors of a given process
Co-cristallisation: active/inactive conformation
Cancer
Inflammation
Infectiology …

2. High throughput screening (HTS)
Caroline BARETTE
Cell incubator
The robotic instrumentation
in P2 safety level
Multimode Reader
(Abs., lumi,
Fluo: FP, HTRF®)
96-channel
pipeting head
Cell washer
Help in the design of miniaturized assays
Robotization
(adaptation and programming)
Automated Screening
Hit-picking
Secondary screen
& in cellulo characterization (HCS)
SAR
> compounds
-20°C, DMSO
Compounds storage and management
Assay miniaturization and automation
Chemicals
Cells or proteins…
Signal detection

3. automated hit-picking
In house computing
Céline CHARAVAY (GIPSE)
TAMIS: Tool to Analyze and Manage Information of Screening
Threshold
Hit selection and
automated hit-picking
Statistical
parameters
R. NADON
McGill Univ.,
Montreal
Compounds libraries management
Screening data analysis
Statistical normalizations
Comparative analysis of screens
Quality Approach
Secondary screens
SAR
Chemical synthesis

4. High Content Screening (HCS) / High Content Analysis (HCA)
Emmanuelle SOLEILHAC
The automated imaging systems
Cell by cell analysis
Data analysis
Nuclei & Cell segmentation
Statistical analysis
coll. Pr Robert NADON
McGill University, Montreal
© Martinez & Soleilhac
ArrayScan
IncuCyte
Staining 1
Staining 2
Staining 3
2880 images/plate or more
Applications
Validation of bioactive hits (secondary screens)
Dose-response effect
Structure-Activity Relationship (SAR)
Primary screens of small libraries

5. CMBA commitment and success stories IBiSA label and support
2 Research Engineers
1 Senior Scientist
IBiSA label and support
45 projects completed
26 publications & 10 patents
Occasional provisions of service
Depository of « Chimiothèque Nationale » ( compounds)
Commercial libraries
Success stories at BIG:
Liminib
(L. Lafanechère)
COB223
(J.-J. Feige)
CK2 inhibitors
(C. Cochet)
Sterol metabolism in microalgae
(E. Maréchal)
USP2 inhibitors
(M.-O. Fauvarque)
and others,…
30 ongoing or achieved projects
17 publications & 6 patents
Occasional provisions of service
Depository of the French « Chimiothèque Nationale » ( compounds)
Services
R&D
Scientific animation
HCS/HCA
High Content Screening
and Analysis
HTS
High Throughput
Screening

6. Further information Contact people
Contact people
Marie-Odile Fauvarque Tel:
Caroline Barette Tel:
Emmanuelle Soleilhac Tel: