3. Institut Sains Biologi
Muzium Zoologi
Institut Sains Biologi
Museum of Zoology

4. museumzoology.um.edu.my

5. Bulletin of the Museum of Zoology
Next issue end of April 2013

6. Biodiversity seminars

7. Special exhibition

8. Workshop

9. Hunt for Vampire Moth

11. Biodiversity Genomics Lab
With capacity for routine DNA barcoding of all animal groups

12. DNA barcoding of bats
Funded by UMRG158/12SUS: Butterflies of Peninsula Malaysia Project

13. Testing the utility of DNA barcoding for rapid and accurate assessment of bat diversity in Malaysia in the absence of formally described species
John James Wilson*, Sing Kong Wah*, Muhammad Rasul Abdullah Halim, Rosli bin Ramli, Rosli bin Hashim, & Mohd Sofian-Azirun Genetics and Molecular Research, in press

14. How to Rapidly Accelerate Biodiversity Inventory in Regions where Most of the Species are Unknown? Malaysia’s First DNA Barcoding Bioblitz John James Wilson, Kong Wah Sing, Khairunnisa Syaripuddin …..in preparation
Funded by UMRG158/12SUS: Butterflies of Peninsula Malaysia Project

15. Time taken for completion of DNA barcoding Bioblitz
Step
Time taken (hrs)
Specimen collection 12
Specimen sorting 2
DNA extraction
0.5
PCR set-up
PCR 3
PCR check
1.5
2nd PCR set-up
DNA sequencing 48
Sequence editing, alignment, upload 1
Analysis of DNA barcodes
TOTAL
77.5 (3 days)

16. Cost of DNA barcoding Bioblitz
Step
Details of consumables/reagents
Cost of consumables/reagents
/service per specimen in MYR
Specimen collection
1.5ml microcentrifuge tubes
0.07
Specimen sorting
96-well plate
0.14
DNA extraction
Xytogen kits (100 preps), Pipette tips
2.33
PCR
PCR Master mix, Primers, ddH20, 0.2ml PCR tube, Pipette tips
0.95 (0.95)
PCR check
Agarose, TAE buffer, DNA ladder (0.5μl), GelRed DNA stain
1.00 (1.00)*
DNA sequencing
21.00
TOTAL
24.49 (26.49)

17. Building a DNA barcode reference library for the true butterflies (Lepidoptera) of peninsula Malaysia: What about the subspecies?
John-James Wilson1,2, Kong-Wah Sing1, Mohd Sofian-Azirun2
PLoS ONE, in review
Funded by UMRG158/12SUS: Butterflies of Peninsula Malaysia Project

18. Figure 5.