1. From: Pseudomonas Keratitis: Protease IV Gene Conservation, Distribution, and Production Relative to Virulence and Other Pseudomonas Proteases
Invest. Ophthalmol. Vis. Sci ;45(2): doi: /iovs
Figure Legend:
PCR analysis of genomic DNA from P. aeruginosa and other Pseudomonas species for the presence of the protease IV gene. Genomic DNA (500 ng) from 24 P. aeruginosa strains and six non-aeruginosa strains of Pseudomonas was used as a template in PCR reactions containing three different 5′ primer combinations specific for three regions of the protease IV gene; Primer sets A, B, and C yielded products equivalent to three-quarters, one half, and one quarter of the gene, respectively. Numbers are those above each primer set: 1, PA103-29; 2, PAO1; 3, PA103; 4, PA103-AP1; 5, BR-1; 6, PA ALC1; 7, PA ALC2; 8, PA ALC3; 9, PA23; 10, PA70; 11, PA178; 12, PA13004; 13, PA13010; 14, PA13014; 15, PA13036; 16, PA30009; 17, PA30099; 18, PA30132; 19, PA30244; 20, PA51036; 21, PA51037; 22, PA51003; 23, PA51005; 24, PA51131; 25, P. alcaligenes; 26, P. mendocina; 27, P. putida; 28, P. otitidis 10150; 29, P. stutzeri 17001; 30, P. stutzeri The molecular weight marker is φX174 DNA-HaeIII digest; from top to bottom: 1353, 1078, 872, 603, and 310 bases.
Date of download: 10/21/2017
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