1. Nutritional Classes of Microorganism
Agustin Krisna Wardani

2. Nutritional Classification of microorganism
depend on : - source of carbon - how to generate energy ( ATP and reducing power)

3. Carbon sources:
Heterotroph – must obtain carbon in an organic form made by other living organisms such as proteins, carbohydrates, lipids and nucleic acids
Autotroph - an organism that uses CO2, an inorganic gas as its carbon source
not nutritionally dependent on other living things 3

4. Organic nutrients- contain carbon and hydrogen atoms and are usually the products of living things
methane (CH4), carbohydrates, lipids, proteins, and nucleic acids
Inorganic nutrients– atom or molecule that contains a combination of atoms other than carbon and hydrogen
metals and their salts (magnesium sulfate, ferric nitrate, sodium phosphate) 4

7. Non inorganic compound (CO2) chemoautotroph photoautotrophs
Source of energy (ATP)
Source of carbon
Chemical energy
Light energy
Organic compound
chemoheterotroph
photoheterotrophs
Non inorganic compound (CO2)
chemoautotroph
photoautotrophs

8. 8

9. Types of -trophs

10. 10

11. Formation Precursor Metabolites by autrotrophs
Make precursor from CO2 create organic molecules from an inorganic gas.
ex: plants (as autotrophs) use CO2 to create organic molecules to grow.
Have special metabolic pathway (not found in heterotrophs) Calvin-Benson cycle
I molecule CO2  organic molecule (phosphoglyceraldehide)
2 precursor metabolites is produced
10 precursor metabolites from glycolysis, TCA cycle, PP

12. No ATP and reducing power (they need 3 ATP and 2 NADPH for CO2 fixed)  thus, another way to generate ATP
ATP generate from sunlight (photoautotrophs) and inorganic compound (chemoautotrophs)

15. Autotrophs
Central metabolism + Calvin-Benson cycle  12 precursor metabolites

16. Photoautotrophs Generate ATP from photosynthesis:
1. Oxygenic (oxygen producing) photosynthesis
2. Anoxygenic (not oxygen-producing)photosynthesis
Chlorophyl  convert light energy into chemical energy

17. Anoxygenic photosynthesis
Light energy  activate the electron from chlorophyll  flow through electron transport  ATP
Electron rejoin the chlorophyll in unactivated state
Chlorophyll served as electron donor and electron acceptor cyclic photophosphorylation

19. Oxygenic photsynthesis
Generate ATP and reducing power from light energy by process of oxygenic photosynthesis.
2 different chlorophyll molecules (photosystem I and II)
Water is also source of electron
Step 1: Light chlorophill (in Photosystem II) electron transport system  ATP 
Step 2: electron joined to photosystem I  light  2nd transport system  NADPH

21. Chemoautotrops By oxidation of several inorganic compound
They remove electron from inorganic substrate  transport system  ATP
Chemoautotrophs
Substrate
product
Hydrogen bacteria H2 H+
Sulfur bacteria
H2S, S, SO32-
Sulfate
Nitrite oxidizer
NO2- (nitrite ion)
NO3- (nitrate ion)
Iron bacteria
F2+
Fe3+