1. FIG. 4. (A and B) Microtubule-associated protein-2 (MAP2)/leukemia inhibitory factor (LIF) double-staining immunohistochemistry in the periischemia region at 12 hours of reperfusion. Neurons stained with MAP2 (A; fluorescein isothiocyanate) consistently expressed LIF immunoreactivity (B; diaminobenzidine). Arrows point to colocalization of MAP2 and LIF in the two sections. Blood vessels (asterisks) were used as markers to locate the identical area in the two sections. (C and D) Glial fibrillary acidic protein (GFAP)/LIF double-staining immunohistochemistry around the surface of the infarcted cortex at 96 hours of reperfusion. Some of the proliferated astrocytes labeled with GFAP (C; fluorescein isothiocyanate) expressed LIF (D; diaminobenzidine). Arrows point to co-localization of GFAP and LIF in the two sections. (E and F) Isolectin-B4 from G. simplicifolia seeds (GSAI-B4)/LIF double-staining immunohistochemistry in the ischemic core at 48 hours of reperfusion. The distribution of reactive microglia and monocytes/macrophages detected by GSAI-B4 (E; fluorescein isothiocyanate) was different from that of LIF (F; diaminobenzidine). (G to I) LIF/interleukin-6 (IL-6) double-staining immunohistochemistry in the periischemia region at 24 hours of reperfusion. LIF was visualized with Cy2-conjugated anti-goat IgG (G). Interleukin-6 was visualized with Texas Red-conjugated anti-rabbit IgG (H). On superimposed photograph (I), arrowheads point to the co-localization of LIF and IL-6. Bar = 50 μm.
Published in: Shigeaki Suzuki; Kortaro Tanaka; Shigeru Nogawa; Daisuke Ito; Tomohisa Dembo; Arifumi Kosakai; Yasuo Fukuuchi; J Cereb Blood Flow Metab  20,
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