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ENZYMES
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WHAT IS AN ENZYME? organic catalyst
catlalyst - assists the reaction but remain unchanged when the reaction is over. Reusable each chemical reaction requires one enzyme
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modify the rate of reactions structure
large complex proteins consisting of one or more polypeptide chains whose names end in –ase substrate – substance worked on by enzyme
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ENZYME SUBSTRATE maltase maltose lipase lipids protease proteins
amylase amylose
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protein nature – either all protein or proteinc with non-protein parts, called coenzymes – also known as VITAMINS active site – formation of pockets into which reacting molecules fit
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HOW DO ENZYMES WORK? 2 MODELS OF ENZYME FUNCTION
ENZYME-SUBSTRATE COMPLEX “LOCK AND KEY” MODEL
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ENZYME SUBSTRATE COMPLEX
enzyme forms a temporary association with a substance whose reaction rate is affected close physical association between enzyme and substrate is formed enzyme-substrate complex
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enzyme action takes place in enzyme-substrate complex
upon completion of reaction, enzyme and product(s) separate, enzyme is available for another reaction
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LOCK AND KEY MODEL particular enzyme will usually only form a complex with one particular type of substrate
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WHAT INFLUENCES ENZYMES?
TEMPERATURE AMOUNT OF ENZYME & SUBSTRATE pH
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TEMPERATURE reaction rate increases until an optimum temperature
enzyme action rate decreases At high temperatures, enzymes denature
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AMOUNT OF ENZYME & SUBSTRATE
when an excess of substrate is added, the rate of enzyme action increases to a point then remains fixed as long as the enzyme concentration remains constant
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pH enzymes work best at different pH ranges
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**a pH of 7 provides the optimum environment**
acids - high H+ concentration which means LOW pH – below 7 bases – low H+ concentration which means HIGH pH – above 7
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