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ENZYME ACTIVITY How do enzymes work? (NEXT SLIDE).

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Presentation on theme: "ENZYME ACTIVITY How do enzymes work? (NEXT SLIDE)."— Presentation transcript:

1 ENZYME ACTIVITY How do enzymes work? (NEXT SLIDE)

2 DON’T FORGET As we go through this powerpoint, don’t forget to answer the questions your hand-out. (NEXT SLIDE)

3 ENZYME REVIEW ENZYMES ARE MADE OF: CARBOHYDRATE PROTEIN LIPID
NUCLEIC ACID

4 ENZYME REVIEW TRY AGAIN ENZYMES ARE MADE OF: CARBOHYDRATE PROTEIN
LIPID NUCLEIC ACID

5 ENZYME REVIEW TRY AGAIN ENZYMES ARE MADE OF: CARBOHYDRATE PROTEIN
LIPID NUCLEIC ACID

6 ENZYME REVIEW TRY AGAIN ENZYMES ARE MADE OF: CARBOHYDRATE PROTEIN
LIPID NUCLEIC ACID

7 ENZYME REVIEW RIGHT!! ENZYMES ARE MADE OF: CARBOHYDRATE PROTEIN LIPID
NUCLEIC ACID

8 What do enzymes do? Enzymes are a special type of protein. There are many different enzymes in your body. These enzymes perform a variety of different functions (they are the “machines” of your cells). Some of the functions of enzymes are to digest your food and to repair different parts of the cell. How many types of enzymes are there in your body? Lots and lots Only a few

9 What do enzymes do? Enzymes are a special type of protein. There are many different enzymes in your body. These enzymes perform a variety of different functions (they are the “machines” of your cells). Some of the functions of enzymes are to digest your food and to repair different parts of the cell. How many types of enzymes are there in your body? Lots and lots Only a few

10 NOPE, TRY AGAIN Nope, try again

11 AMYLASE Amylase is an enzyme that is found in your saliva. It functions to begin breaking down starch in your food. Click on the body part where you would find the amylase enzyme.

12 AMYLASE Amylase is an enzyme that is found in your saliva. It functions to begin breaking down starch in your food. Click on the body part where you would find the amylase enzyme.

13 AMYLASE Amylase is an enzyme that is found in your saliva. It functions to begin breaking down starch in your food. Click on the body part where you would find the amylase enzyme.

14 RIGHT!! Right!!

15 Enzymes are specific for their targets
Every enzyme (including amylase) is specific for the molecule it acts on. Amylase only breaks down starch. It will not break down lipids or proteins. This is because only certain molecules fit into a given enzyme.

16 Click on the macromolecule that will fit into the red part of the amylase enzyme
STARCH PROTEIN LIPID AMYLASE

17 You really think so?? STARCH Try again.. PROTEIN LIPID AMYLASE

18 You really think so?? STARCH PROTEIN LIPID AMYLASE Try again..

19 RIGHT!! PROTEIN STARCH LIPID AMYLASE

20 STARCH What type of nutrient is STARCH? CARBOHYDRATE PROTEIN LIPID
NUCLEIC ACID

21 STARCH TRY AGAIN What type of nutrient is STARCH? CARBOHYDRATE PROTEIN
LIPID NUCLEIC ACID

22 STARCH TRY AGAIN What type of nutrient is STARCH? CARBOHYDRATE PROTEIN
LIPID NUCLEIC ACID

23 STARCH TRY AGAIN What type of nutrient is STARCH? CARBOHYDRATE PROTEIN
LIPID NUCLEIC ACID

24 STARCH RIGHT!! What type of nutrient is STARCH? CARBOHYDRATE PROTEIN
LIPID NUCLEIC ACID

25 STARCH In the lab scientists can test for nutrients in different foods. One of these 4 tests is the correct test for the presence of STARCH. 2-3 drops of iodine; Blue-black if starch Is present 2-3 mL of Biuret; Purple if starch is present Chemically-treated Paper; green or brown If starch is present Brown paper; paper Becomes translucent In presence of starch

26 TRY AGAIN Recall the lab where we tested for the nutrients in different foods. How did we test for the presence of STARCH? 2-3 drops of iodine; Blue-black if starch Is present 2-3 mL of Biuret; Purple if starch is present Chemically-treated Paper; green or brown If starch is present Brown paper; paper Becomes translucent In presence of starch

27 TRY AGAIN Recall the lab where we tested for the nutrients in different foods. How did we test for the presence of STARCH? 2-3 drops of iodine; Blue-black if starch Is present 2-3 mL of Biuret; Purple if starch is present Chemically-treated Paper; green or brown If starch is present Brown paper; paper Becomes translucent In presence of starch

28 TRY AGAIN Recall the lab where we tested for the nutrients in different foods. How did we test for the presence of STARCH? 2-3 drops of iodine; Blue-black if starch Is present 2-3 mL of Biuret; Purple if starch is present Chemically-treated Paper; green or brown If starch is present Brown paper; paper Becomes translucent In presence of starch

29 RIGHT!! RIGHT!! Now, click the beaker to add 3 drops iodine to the solution to see if it contains starch:

30

31 So… is there starch in the solution?
YES!! NO!!

32 TRY AGAIN So… is there starch in the solution? YES!! NO!!

33 RIGHT!! RIGHT!! And… is there a carbohydrate in the solution? YES!! NO!!

34 TRY AGAIN RIGHT!! So… is there a carbohydrate in the solution? YES!! NO!!

35 RIGHT!! Right!!

36 BACK TO AMYLASE Let’s do an experiment. Let’s test the affect of amylase on starch. The two beakers below contain starch. Click on the beaker on the left to add amylase enzyme.

37 BACK TO AMYLASE In reality, enzymes are microscopic (too small to see). In this simulation, green stars will represent the amylase enzyme. Click on each beaker to add iodine.

38 BACK TO AMYLASE When you add iodine to the beaker with the amylase, it does not turn blue-black. It turns yellow (the color of the iodine). Click on the other beaker to add iodine.

39 Which solution contains starch?
Click on the beaker that CONTAINS starch

40 BACK TO AMYLASE NOPE, no starch in this one..

41 RIGHT!! Why doesn’t the left-hand beaker contain starch?
Iodine doesn’t react with amylase Amylase broke down the starch, so there is none left to react with the iodine

42 BACK TO AMYLASE TRY AGAIN NOPE: amylase doesn’t affect iodine.
Iodine doesn’t react with amylase Amylase broke down the starch, so there is none left to react with the iodine

43 RIGHT!! Right!!

44 Where does the starch go?
Ok, so amylase breaks down starch. Once starch is broken down, iodine can’t detect it. But, what happens to the starch? Click the beaker to do another test.

45 Where does the starch go?
Let’s do the test for sugar. If the chemically treated paper turns green or brown, there is sugar in the solution. Click either of the strips next to the beakers to see the results.

46 Where does the starch go?
Click on the beaker that contains SUGAR.

47 Where does the starch go?
NOPE, no sugar in that one…

48 RIGHT!! Right!!

49 THE REACTION AMYLASE breaks down STARCH into its subunits, which are sugars. SUGAR STARCH AMYLASE

50 STARCH is called the SUBSTRATE SUGAR is called the PRODUCT
AMYLASE In this reaction, STARCH is called the SUBSTRATE SUGAR is called the PRODUCT AMYLASE ENZYME is called the CATALYST

51 Enzyme Reactions - Substrate
This means that a SUBSTRATE is: The thing that gets broken down The thing that you end up with Something that performs a reaction, but isn’t used up GO BACK TO REACTION

52 Enzyme Reactions - Substrate
TRY AGAIN Enzyme Reactions - Substrate This means that a SUBSTRATE is: The thing that gets broken down The thing that you end up with Something that performs a reaction, but isn’t used up GO BACK TO REACTION

53 Enzyme Reactions - Substrate
TRY AGAIN Enzyme Reactions - Substrate This means that a SUBSTRATE is: The thing that gets broken down The thing that you end up with Something that performs a reaction, but isn’t used up GO BACK TO REACTION

54 RIGHT!! Right!!

55 Enzyme Reactions - Product
This means that a PRODUCT is: The thing that gets broken down The thing that you end up with Something that performs a reaction, but isn’t used up GO BACK TO REACTION

56 Enzyme Reactions - Product
TRY AGAIN Enzyme Reactions - Product This means that a PRODUCT is: The thing that gets broken down The thing that you end up with Something that performs a reaction, but isn’t used up GO BACK TO REACTION

57 Enzyme Reactions - Product
TRY AGAIN Enzyme Reactions - Product This means that a PRODUCT is: The thing that gets broken down The thing that you end up with Something that performs a reaction, but isn’t used up GO BACK TO REACTION

58 RIGHT!! Right!!

59 Enzyme Reactions - Catalyst
This means that a CATALYST is: The thing that gets broken down The thing that you end up with Something that performs a reaction, but isn’t used up GO BACK TO REACTION

60 Enzyme Reactions - Catalyst
TRY AGAIN Enzyme Reactions - Catalyst This means that a CATALYST is: The thing that gets broken down The thing that you end up with Something that performs a reaction, but isn’t used up GO BACK TO REACTION

61 Enzyme Reactions - Catalyst
TRY AGAIN Enzyme Reactions - Catalyst This means that a CATALYST is: The thing that gets broken down The thing that you end up with Something that performs a reaction, but isn’t used up GO BACK TO REACTION

62 RIGHT!! Right!!

63 Is PROTEIN the… AMINO ACIDS PROTEIN PROTEINASE PRODUCT SUBSTRATE
CATALYST

64 Did you understand? TRY AGAIN AMINO ACIDS PROTEIN PROTEINASE PRODUCT
SUBSTRATE CATALYST

65 Did you understand? TRY AGAIN AMINO ACIDS PROTEIN PROTEINASE PRODUCT
SUBSTRATE CATALYST

66 Is PROTEINASE the… AMINO ACIDS PROTEIN PROTEINASE PRODUCT SUBSTRATE
CATALYST

67 Did you understand? TRY AGAIN AMINO ACIDS PROTEIN PROTEINASE PRODUCT
SUBSTRATE SUBSTRATE CATALYST

68 Did you understand? TRY AGAIN AMINO ACIDS PROTEIN PROTEINASE PRODUCT
SUBSTRATE SUBSTRATE CATALYST

69 Are AMINO ACIDS the… AMINO ACIDS PROTEIN PROTEINASE PRODUCT SUBSTRATE
CATALYST CATALYST

70 Did you understand? TRY AGAIN AMINO ACIDS PROTEIN PROTEINASE PRODUCT
SUBSTRATE SUBSTRATE CATALYST CATALYST

71 Did you understand? TRY AGAIN AMINO ACIDS PROTEIN PROTEINASE PRODUCT
SUBSTRATE SUBSTRATE CATALYST CATALYST

72 Way to go!!

73 ENZYME NAMES AMYLASE PROTEINASE YES, I DID NO, I DIDN’T
Did you notice something about the names of the two enzymes: AMYLASE PROTEINASE YES, I DID NO, I DIDN’T

74 ENZYME NAMES RIGHT! Both names end in ASE AMYLASE PROTEINASE

75 ENZYME NAMES Look closely! Both names end in ASE AMYLASE PROTEINASE

76 DID YOU UNDERSTAND? LIPIDASE is probably: An enzyme that breaks
Down lipids An enzyme that Repairs cells A sugar that is found In lipids A type of lipid

77 NOPE! TRY AGAIN

78 RIGHT!! Right!!

79 Think about this… As time goes on, what happens to the amount of SUBSTRATE? SUGAR STARCH AMYLASE

80 We can test it using our starch test (iodine)
Each beaker contains starch. Amylase enzyme was added at 0 seconds. Click on each beaker below to add the iodine. 0 sec 20 sec 40 sec 60 sec 120 sec

81 We can test it using our starch test (iodine)
Each beaker contains starch. Amylase enzyme was added at 0 seconds. Click on each beaker below to add the iodine. 0 sec 20 sec 40 sec 60 sec 120 sec Lots of starch is still left after 0 seconds.

82 We can test it using our starch test (iodine)
Each beaker contains starch. Amylase enzyme was added at 0 seconds. Click on each beaker below to add the iodine. 0 sec 20 sec 40 sec 60 sec 120 sec Some starch is left after 20 seconds. Amylase has broken down some starch into sugar

83 We can test it using our starch test (iodine)
Each beaker contains starch. Amylase enzyme was added at 0 seconds. Click on each beaker below to add the iodine. 0 sec 20 sec 40 sec 60 sec 120 sec Very little starch is left after 40 seconds. Amylase has broken down almost all of the starch into sugar

84 We can test it using our starch test (iodine)
Each beaker contains starch. Amylase enzyme was added at 0 seconds. Click on each beaker below to add the iodine. 0 sec 20 sec 40 sec 60 sec 120 sec No starch is left after 60 seconds. Amylase has broken down all the starch into sugar. The yellow color is from the iodine.

85 We can test it using our starch test (iodine)
Each beaker contains starch. Amylase enzyme was added at 0 seconds. Click on each beaker below to add the iodine. 0 sec 20 sec 40 sec 60 sec 120 sec No change between 60 and 120 seconds. Amylase already broke down all the starch into sugar.

86 We can show this as a graph
What should go on the X axis? Amount of enzyme TIME (in seconds)

87 We can show this as a graph
What should go on the X axis? Amount of enzyme TIME (in seconds)

88 We can show this as a graph
Amount of enzyme What should go on the Y axis? (HINT: This is what we are MEASURING) Amount of substrate RIGHT! Time usually goes on the X axis 20 40 60 120 Time (in seconds)

89 We can show this as a graph
Amount of enzyme What should go on the Y axis? (HINT: This is what we are MEASURING) Adding iodine doesn’t tell us how much ENZYME is in the beaker. Amount of substrate 20 40 60 120 Time (in seconds)

90 We can show this as a graph
RIGHT!! The amount of substrate should go on the Y axis. Amount of substrate REMEMBER!! STARCH is the SUBSTRATE. Dark blue-black means there is MORE STARCH. SUBSTRATE increases as you go up the Y axis 20 40 60 120 Time (in seconds)

91 Let’s GRAPH!! Amount of substrate 20 40 60 120 Time (in seconds)

92 SUMMARIZE THE GRAPH As time goes on, the amount of SUBSTRATE (starch)
INCREASED DECREASED

93 SUMMARIZE THE GRAPH As time goes on, the amount of SUBSTRATE (starch)
TRY AGAIN SUMMARIZE THE GRAPH As time goes on, the amount of SUBSTRATE (starch) INCREASED DECREASED

94 SUMMARIZE THE GRAPH As time goes on, the amount of SUBSTRATE (starch) DECREASED because Starch was broken down by amylase Iodine made starch turn into sugar

95 TRY AGAIN SUMMARIZE THE GRAPH As time goes on, the amount of SUBSTRATE (starch) DECREASED because Starch was broken down by amylase Iodine made starch turn into sugar

96 SUMMARIZE THE GRAPH As time goes on, the amount of SUBSTRATE (starch) DECREASED because the amylase enzyme digested the starch and made it into sugar.

97 So… Which graph shows the amount of substrate going to zero faster?
Time (sec) Time (sec)

98 TRY AGAIN So… Nope, the amount of substrate gets to zero in less time in the OTHER graph. Substrate Substrate Time (sec) Time (sec)

99 RIGHT!! So… YES!! The amount of substrate gets to zero in less time in this graph. Substrate Substrate Time (sec) Time (sec)

100 So… Which enzyme works FASTER? Substrate Substrate Time (sec)

101 TRY AGAIN So… Nope, not this one. The faster the enzyme works, the faster the substrate will decrease. Time (sec) Substrate Substrate Time (sec)

102 RIGHT!! So… YES!! This enzyme works faster because the substrate decreases faster!! Time (sec) Substrate Substrate Time (sec)

103 Look at the two reactions in the beakers and the two graphs
Look at the two reactions in the beakers and the two graphs. The top set of beakers goes with which graph? Substrate Time (sec) Substrate Time (sec)

104 NOPE, the reaction in the top set of beakers took LONGER to finish
Substrate Time (sec) Substrate Time (sec)

105 RIGHT. The reaction in the top set of beakers took longer to finish
RIGHT!! The reaction in the top set of beakers took longer to finish. The reaction in the bottom set finished faster. Substrate Time (sec) Substrate Time (sec)

106 Which of the ENZYMES worked FASTER?
Substrate Time (sec) Substrate Time (sec)

107 Which of the ENZYMES worked FASTER
Which of the ENZYMES worked FASTER? Nope, this reaction took longer, so this enzyme was slower. Time (sec) Substrate Substrate Time (sec)

108 RIGHT!! RIGHT!! This reaction happened FASTER, so the enzyme worked FASTER. Substrate Time (sec) What might be the difference Between the top and bottom sets Of beakers?

109 What do you think can affect the speed of an enzyme?
TEMPERATURE pH AMOUNT OF LIGHT

110 What do you think can affect the speed of an enzyme?
TEMPERATURE RIGHT!! Changing the pH of the reaction can affect the speed of an enzyme. ANYTHING ELSE? pH AMOUNT OF LIGHT

111 What do you think can affect the speed of an enzyme?
TEMPERATURE RIGHT!! Changing the temperature of the reaction can ALSO affect the speed of an enzyme. pH AMOUNT OF LIGHT

112 What do you think can affect the speed of an enzyme?
TEMPERATURE RIGHT!! Changing the temperature of the reaction can affect the speed of an enzyme. ANYTHING ELSE? pH AMOUNT OF LIGHT

113 What do you think can affect the speed of an enzyme?
TEMPERATURE RIGHT!! Changing the pH of the reaction can ALSO affect the speed of an enzyme. pH AMOUNT OF LIGHT

114 What do you think can affect the speed of an enzyme?
TEMPERATURE Sorry, most enzymes are NOT affected by the amount of light… Pick again pH AMOUNT OF LIGHT

115 Factors affecting enzyme speed
We can make a graph of enzyme speed at different temperatures.

116 Graph of ENZYME SPEED What should go on the X axis? Speed of enzyme
Temperature (C)

117 Graph of ENZYME SPEED TRY AGAIN
Nope, the speed of the enzyme is what we are MEASURING,it should go on the Y axis. Speed of enzyme Temperature (C)

118 RIGHT! Temperature should go on the X axis.
Graph of ENZYME SPEED Amount of substrate What should go on the Y axis? (HINT: This is what we are MEASURING) Speed of enzyme RIGHT! Temperature should go on the X axis. 10 20 30 40 50 60 Temperature (C)

119 Graph of ENZYME SPEED TRY AGAIN Amount of substrate
What should go on the Y axis? (HINT: This is what we are MEASURING) We’re not measuring the amount of substrate this time. Speed of enzyme 10 20 30 40 50 60 Temperature (C)

120 REMEMBER!! The enzyme is working FASTER
Graph of ENZYME SPEED FAST RIGHT!! The speed of enzyme should go on the Y axis. Speed of enzyme REMEMBER!! The enzyme is working FASTER As you go UP the Y axis. SLOW 10 20 30 40 50 60 Temperature (C)

121 Let’s GRAPH!! FAST Amylase is found in saliva. Click on the red button that is approximately at body temperature Speed of enzyme SLOW 10 20 30 40 50 60 Temperature (C)

122 Let’s GRAPH!! TRY AGAIN Amylase is found in saliva.
FAST Amylase is found in saliva. 5C is close to freezing. TOO COLD!! Speed of enzyme SLOW 10 20 30 40 50 60 Temperature (C)

123 Let’s GRAPH!! TRY AGAIN Amylase is found in saliva.
FAST Amylase is found in saliva. 22C is close to room temperature. TOO COLD!! Speed of enzyme SLOW 10 20 30 40 50 60 Temperature (C)

124 Let’s GRAPH!! TRY AGAIN Amylase is found in saliva. 55C is 131F.
FAST Amylase is found in saliva. 55C is 131F. TOO HOT!! Speed of enzyme SLOW 10 20 30 40 50 60 Temperature (C)

125 Let’s GRAPH!! Amylase is found in saliva. 37C is body temperature.
FAST Amylase is found in saliva. 37C is body temperature. RIGHT!! Amylase will work best at about 37C because it is normally found in your mouth Speed of enzyme SLOW 10 20 30 40 50 60 Temperature (C)

126 At very low temperatures
Let’s GRAPH!! FAST Amylase works slowly At very low temperatures Because it is not close To body temperature Speed of enzyme SLOW 10 20 30 40 50 60 Temperature (C)

127 Let’s GRAPH!! Amylase will work Faster as it gets Closer to body
Temperature. Speed of enzyme SLOW 10 20 30 40 50 60 Temperature (C)

128 Let’s GRAPH!! Amylase will work fastest at body Temperature. FAST
Speed of enzyme The temperature where Amylase works BEST is called the OPTIMUM TEMPERATURE SLOW 10 20 30 40 50 60 Temperature (C)

129 As the temperature gets too hot, Amylase doesn’t work as well.
Let’s GRAPH!! As the temperature gets too hot, Amylase doesn’t work as well. FAST Speed of enzyme SLOW 10 20 30 40 50 60 Temperature (C)

130 Let’s GRAPH!! Click on the OPTIMUM TEMPERATURE FAST Speed of enzyme
SLOW 10 20 30 40 50 60 Temperature (C)

131 Let’s GRAPH!! Click on the OPTIMUM TEMPERATURE RIGHT!! FAST
Speed of enzyme RIGHT!! SLOW 10 20 30 40 50 60 Temperature (C)

132 What is the optimum temperature for this enzyme?
Temperature (C) 20 50 60 10 40 30 Speed of enzyme SLOW FAST 10C 20C 30C

133 What is the optimum temperature for this enzyme?
TRY AGAIN What is the optimum temperature for this enzyme? Temperature (C) 20 50 60 10 40 30 Speed of enzyme SLOW FAST REMEMBER: The OPTIMUM is the temperature the enzyme works fastest at. 10C 20C 30C

134 What is the optimum temperature for this enzyme?
TRY AGAIN What is the optimum temperature for this enzyme? Temperature (C) 20 50 60 10 40 30 Speed of enzyme SLOW FAST REMEMBER: The OPTIMUM is the temperature the enzyme works fastest at. 10C 20C 30C

135 RIGHT!! Right!!

136 What is the optimum pH for this enzyme?
FAST 9 Speed of enzyme 10 11 SLOW 2 4 6 8 10 12 pH

137 What is the optimum pH for this enzyme?
TRY AGAIN What is the optimum pH for this enzyme? FAST Look closely… 9 Speed of enzyme 10 11 SLOW 2 4 6 8 10 12 pH

138 What is the optimum pH for this enzyme?
TRY AGAIN What is the optimum pH for this enzyme? FAST Look closely… 9 Speed of enzyme 10 11 SLOW 2 4 6 8 10 12 pH

139 RIGHT!! Right!!

140 What is the optimum temperature for this enzyme?
FAST 20C Speed of enzyme 63C 48C SLOW 20 30 40 50 60 70 80 Temperature (C)

141 What is the optimum temperature for this enzyme?
TRY AGAIN What is the optimum temperature for this enzyme? FAST 20C Speed of enzyme 63C 48C SLOW 20 30 40 50 60 70 80 Temperature (C)

142 What is the optimum temperature for this enzyme?
TRY AGAIN What is the optimum temperature for this enzyme? FAST 20C Speed of enzyme 63C 48C SLOW 20 30 40 50 60 70 80 Temperature (C)

143 RIGHT!! Right!! You rock at this...

144 What temperature does the enzyme work best at?
Time Substrate Temperature = 25C Time Substrate Temperature = 30C Time Substrate Temperature = 35C At 25C At 30C At 35C

145 REMEMBER: the faster the substrate decreases, the faster the enzyme is working
Time Substrate Temperature = 25C Time Substrate Temperature = 30C Time Substrate Temperature = 35C At 25C At 30C At 35C

146 REMEMBER: the faster the substrate decreases, the faster the enzyme is working
Time Substrate Temperature = 25C Time Substrate Temperature = 30C Time Substrate Temperature = 35C At 25C At 30C At 35C

147 TO SHOW HOW WELL YOU UNDERSTOOD, COMPLETE THIS SHORT QUIZ
RIGHT!! TO SHOW HOW WELL YOU UNDERSTOOD, COMPLETE THIS SHORT QUIZ

148 QUIZ - QUESTION 1 SALIVA LIVER STOMACH
Where in your body is the amylase enzyme found? SALIVA LIVER STOMACH

149 QUIZ - QUESTION 1 TRY AGAIN SALIVA LIVER STOMACH
Where in your body is the amylase enzyme found? SALIVA LIVER STOMACH

150 QUIZ - QUESTION 1 TRY AGAIN SALIVA LIVER STOMACH
Where in your body is the amylase enzyme found? SALIVA LIVER STOMACH

151 RIGHT!! Right!!

152 QUIZ - QUESTION 2 Click on the CATALYST in this reaction: SUGAR STARCH
AMYLASE

153 QUIZ - QUESTION 2 TRY AGAIN
REMEMBER: The catalyst performs the reaction, but isn’t used up SUGAR STARCH AMYLASE

154 QUIZ - QUESTION 2 TRY AGAIN
REMEMBER: The catalyst performs the reaction, but isn’t used up SUGAR STARCH AMYLASE

155 RIGHT!! Right!!

156 QUIZ - QUESTION 3 Click on the substrate that is most likely to react with the enzyme below: STARCH PROTEIN LIPID

157 QUIZ - QUESTION 3 TRY AGAIN
REMEMBER: The subtrate that fits into a protein will be the one that reacts with it. STARCH PROTEIN LIPID

158 QUIZ - QUESTION 3 TRY AGAIN
REMEMBER: The subtrate that fits into a protein will be the one that reacts with it. STARCH PROTEIN LIPID

159 RIGHT!! Right!! PROTEIN

160 QUIZ - QUESTION 4 What is the most likely name of the enzyme in question 3 that reacts with protein? PROTEINOSE PROTEINATION PROTEINZYME PROTEINASE

161 QUIZ - QUESTION 4 TRY AGAIN
NOPE: sugars end in -OSE, not enzymes (sucrose, lactose, glucose) PROTEINOSE PROTEINATION PROTEINZYME PROTEINASE

162 QUIZ - QUESTION 4 TRY AGAIN NOPE: think about “lactase” and “amylase”
PROTEINOSE PROTEINATION PROTEINZYME PROTEINASE

163 QUIZ - QUESTION 4 TRY AGAIN NOPE: think about “lactase” and “amylase”
PROTEINOSE PROTEINATION PROTEINZYME PROTEINASE

164 RIGHT!! Right!! PROTEINASE

165 QUIZ - QUESTION 5 Click on the part of the graph that shows the OPTIMAL temperature for this enzyme Enzyme Activity Temperature (C)

166 QUIZ - QUESTION 5 TRY AGAIN
REMEMBER: the OPTIMAL temperature for an enzyme is the temperature where the enzyme works BEST (highest activity) Enzyme Activity Temperature (C)

167 RIGHT!! Right!!

168 QUIZ - QUESTION 6 Click on the graph that shows the optimum pH for this enzyme. pH = 5.0 pH = 7.0 pH = 3.0 Substrate Substrate Substrate Time Time Time

169 QUIZ - QUESTION 6 TRY AGAIN
REMEMBER: Substrate decreases in LESS time if the enzyme works faster pH = 5.0 pH = 7.0 pH = 3.0 Substrate Substrate Substrate Time Time Time

170 QUIZ - QUESTION 6 TRY AGAIN
REMEMBER: Substrate decreases in LESS time if the enzyme works faster pH = 5.0 pH = 7.0 pH = 3.0 Substrate Substrate Substrate Time Time Time

171 RIGHT!! Right!!

172 RIGHT!! Right!!

173 You have successfully completed the enzyme PowerPoint.
CONGRATULATIONS! Finish the analysis questions in your packet.


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