1. Enabling healthier, sustainable aquafeeds

2. Ideal Traits of a Novel Alternative Protein
Trait Metric
Protein content ≥ Fish meal
Amino acid content Fish meal
Performance Growth ≥ fish meal diets
Palatability Comparable taste, texture, appearance
Cost Competitive with fish meal
Ease of use Traceable, scalable, safe
Sustainability Non-food chain, resource efficient
Immuno-nutrition Disease resistance & other benefits

3. Protein Content
Fish Meal
2014
2016
2018

4. AA profile in KBM resembles FM through strain improvement
Amino Acid Ratios
AA profile in KBM resembles FM through strain improvement
Fish meal target

5. Performance TROUT 5% KBM 10% KBM Growth of finfish supported
Control
(Standard)
5% KBM
10% KBM
Growth (g/fish)
220 ±1
217 ±7
205±5
Specific growth rate
(SGR, %/d)
3.24±0.01
3.21±0.03
3.16±0.02
Feed conversion ratio
0.85±0.01
0.82±0.01
0.84±0.00
Survival (%)
96.2±0.9b
98.9±0.9ab
100a
Protein efficiency ratio
(PER)
2.42±0.04
2.49±0.02
2.44±0.01
Growth of finfish supported
Equivalent or better:
Growth rate
Survival
FCR
SERIOLA
Control
(Standard) 5%
KBM
Growth (g) ± ±
Survival
100
FCR
1.76
1.66

6. From “Feed to Fork” – Kampachi, a sashimi-grade yellowtail
Palatability
>70 panelists could NOT detect a taste difference
From “Feed to Fork” – Kampachi, a sashimi-grade yellowtail

7. Cost of Manufacturing Costs decrease due to:
Economies of scale
Tech improvements
Use of waste streams as feedstock
~2020, price parity with fish meal
Price of Fish meal

8. Pre-Commercial/Commercial
Scalability
Bench/Lab 1L
( )
Pilot
1500L
( )
Pre-Commercial/Commercial
Up to 20,000 = 50,000L+
( )

9. Commercial Manufacturing
An opportunity to co-locate with existing biorefineries
Biorefinery streams for KBM
Beer
Various cuts of hydrous ethanol, fusel oils
Syrup / thin stillage
Blended/complement DDGS

10. Sustainability Small land & water footprint Avoid marine resources
Efficient resource use
Non-competition for human food
Feedstock flexible
Commitment to stewardship

11. KnipBio’s Immuno-Nutrition Strategy
Biotechnology enables benefits beyond protein
Improved survival
Carotenoids
Biomolecules (taurine)
Specialty molecules
Protein
KnipBio Meal (KBM)

12. Engineered KBM Provides Lost Nutrients
Taurine deficiencies in FM-free diets can lead to poor performance
Essential in aquaculture (larvae)
Taurine deficiency

13. EMS/AHPND confirmed (Severity grade) in hour post-infection
Improved Survival
“C”
Negative control
Positive control
“A”
“B”
Treatment
EMS/AHPND confirmed (Severity grade) in hour post-infection
72-96
AVG
T1 (KB “A”)
G0-1
1.33
T2 (KB “B”)
G1-2
1.22
T3 (KB “C”)
G1-3
1.83
Positive control (T4)
G1-4
2.07
Negative control (T5) -
~33% improved survival

14. Advanced Carotenoid Technology
Target

15. Summary Biotechnology enables a versatile commercial solution
Trait Metric
Protein content Completed
Amino acid content Ideal ratio nearly complete
Performance Demonstrated
Palatability No off-flavors
Cost Reduction program on-track
Ease of use Traceable, integrated supply chain
Sustainability Efficient, flexible, long-term
Immuno-nutrition Benefits beyond “just” protein
Company Confidential

16. Contact: lfeinberg@knipbio.com
@knipbio facebook.com/knipbio
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