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From: The Rd8 Mutation of the Crb1 Gene Is Present in Vendor Lines of C57BL/6N Mice and Embryonic Stem Cells, and Confounds Ocular Induced Mutant Phenotypes.

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Presentation on theme: "From: The Rd8 Mutation of the Crb1 Gene Is Present in Vendor Lines of C57BL/6N Mice and Embryonic Stem Cells, and Confounds Ocular Induced Mutant Phenotypes."— Presentation transcript:

1 From: The Rd8 Mutation of the Crb1 Gene Is Present in Vendor Lines of C57BL/6N Mice and Embryonic Stem Cells, and Confounds Ocular Induced Mutant Phenotypes Invest. Ophthalmol. Vis. Sci ;53(6): doi: /iovs Figure Legend: Genotyping for rd8. (A) Genotyping of vendor mice by PCR for rd8. Bands were resolved by capillary electrophoresis. Mice are from HSD, DCT, CRL, TAC, and JAX. Genotyping for rd8 was performed by PCR. Two representative mice out of 7 obtained from each vendor in 2 separate shipments are shown. (B) Sequencing of Crb1 gene shows a single base deletion in the N substrain at the expected position in the aligned sequences (MegAlign program). N-MF, N substrain sequenced with forward primer; N-MR, N substrain sequenced with reverse primer; J-MF, J substrain sequenced with forward primer; J-MR, J substrain sequenced with reverse primer. Date of download: 10/21/2017 The Association for Research in Vision and Ophthalmology Copyright © All rights reserved.


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