1. Annotation Presentation
Week 2
Basic Information
Module

2. Begin annotating your gene
Pathway Annotation
Log in to IMG-ACT
“Click”
Begin annotating your gene
in lab notebook.

3. Basic Information Module in
Pathway Annotation
Scroll down to
Basic Information Module in
Lab Notebook

4. Then what?
To get to Gene Details page for your gene, return to Gene Cart and click on Gene OID
Scroll down

5. Then what? With Lab Notebook in EDIT mode, copy/paste
requested information
into appropriate boxes.

6. Then what? “CLICK” Copy/paste nucleotide sequence in FASTA format
and record
sequence length (bp)
into boxes.

7. Then what? Copy/paste amino acid sequence in FASTA format
and record the
protein length (aa)
into boxes.
“CLICK”

8. Click on “peptide” link
Then what?
“CLICK”
Under Statistics,
Click on “peptide” link

9. Then what?
Copy/paste
isoelectric point value
into box in lab notebook

10. Recording results in your Lab Notebook
Be sure you SAVE your lab notebook entries

11. Recording results in your Lab Notebook
Helpful hint:
Create a link in your notebook to Gene Details page for your gene for quick reference in later modules