1. EQUIPMENTS

2. Autoclave
Incubator
Centrifuge
Balances
pH Meter
Micropipette
Refrigerators & Freezers
Cryo storage Containers
Cell Counter
Dissecting Microscope

3. AUTOCLAVE Pressure: 15 pound(lb)/inch2 in excess of atm Temp. : 121 C
EXPOSURE for 15 min. sufficient
(sterlized)
PRINCIPLE: Steam under Pressure

4. INCUBATOR Hot Room for Cell Culture Large enough: (~50-200L)
1.5 to 6 ft3
III. Controlled temp.

5. Humid CO2 Incubator Controlled atmosphere
Mixture :Humidity & elevated co2 in Atm
Controlled atmosphere

7. Magnetic Stirrer Rapid action for dissolving Chemicals i.e;
enzymatic tissues Diasaggregation
Suspension cultures

8. Cell-sediment satisfactorily at (80-100g)
CENTRIFUGE
To inc. conc. of cells
Wash off reagent
Cell-sediment satisfactorily at (80-100g)

9. BALANCES
An accurate measure of Weighing Items

10. pH Meter
To Check pH of :
Media
Special reagents

11. Micropipette A Pipettor To pipette conc. (~10-200microL)
Sterilzed tips are used

12. REFRIGERATORS / FREEZERS
Used for Storage
In Hospitals , Blood Banks.
Hygienic cold rooms

13. CRYOSTORAGE CONTAINERS
Storage at extreme low Temp.
Stored in:
35L Freezer
In canes & canisters
In drawers & racks

14. CELL COUNTER To precise quantitative growth kinetics
Automated cell counting
Size analysis

16. DISSECTING MICROSCOPE
Dissecting Tissue’s small pieces
Counting & picking of monolayer colonies

17. Hot-Plate Magnetic Stirrer
To accelerate dissolutions of some reagents