1. Combinations of oligonucleotides ISTR
GENETICS VARIABILITY ESTIMATION AMONG MANGIFERA ACCESSIONS IN CUBA USING ISTR MARKERS
Izquierdo, M1 ,Capote del Sol, M2, Valdés , L. A2, Valdés-Infante J2, Coto, O2, Pérez, L3, Ignacia de la Cruz, M3
1Center for Protein Studies , Faculty of Biology, University of Havana, Cuba
2Research institute in tropical fruity, Havana, Cuba
3 Plant Biology Department, Faculty of Biology, University of Havana, Cuba
ABSTRACT
Genetic variability studies can be useful for the rational management of material as well as its conservation and improvement. Inverse sequence-tagged repeat (ISTR) is a PCR-based technique which allows
studying individuals and population’s genetic diversity, identifying cultivars, among other applications. The aim of this work was to estimate the genetic variability of a mango progeny using ISTR markers.
The evaluation of mango genotypes with ISTR markers generated 40 polymorphic bands, corroborating the utility of this technique for identification of genetic variability in this specie
Índices y sus abreviaturas
Combinations of oligonucleotides ISTR
F2/B2B
F5/B2B
F3/B3
polymorphic bands(np) 15 10
no polymorphic bands (nnp) 1
total of polymorphic bands (n) 16
Average of polymorphic bands(np (P)
13,6
Number of banding patterns (Tp) 36 9 23
Number of unique banding patterns(Tpu) 31 6 17
Identification percentage(%) (Pi)
86,1
66,6
73,9
Number. average bands patterns (I)
22,6
Number. average unique banding patterns(Iu) 18
expected heterozygosity(Hep)
0,26
0,56
0,46
Average expected heterozygosity(He)
0,42
Figure 2 : Cluster analysis of hybrid selections mango, based on the 41 markers generated ISTR
Concluding Remarks
The three primer combinations detect high polymorphism in the mango progeny.
The unique patterns of bands obtained with the primer combinations F3 / B3 and F5 / B2B allowed to differentiate into genotype located within the same group, resulting promising to determine diversity within the progeny.
Figure 1: Gel Agarose representative ISTR analysis. Genomic DNA from progeny hybrid selections mango cv. 'Julie' With the primer combinations F3 / B3.Carriles 1-50: híbridas1-50 selections; Carril J: cv.'Julie'; Λ lane: molecular weight marker λ (HindIII / EcoRI).
Genotype for the development of cultivars (SH-6, SH-15 and SH-25) and for use as rootstock (SH-38) were obtained.