1. Prof.Dr. Raúl Carlos López Sánchez
Ecophysiological characterization of Coccoloba uvifera L.: the case of a salt and drought stress tolerant plant species for sand dune reclamation in Cuba
Prof.Dr. Raúl Carlos López Sánchez
Local promoter

2. Prof.Dr. Raúl Carlos López Sánchez
Overall Academic Objective (OAO): strengthen research and training on abiotic, especially salt and drought, stress of economically interesting species at Granma University (GU).
Overall Developmental Objective (ODO): improve capacity building for research in salt and drought stress at GU.
Specific Academic Objective (SAO): strengthen research and training on ecophysiological, nutritional and phyto-chemical characterization and fruit production under salt and drought stress of C. uvifera in saline coastal soils of Cuba.
Specific Developmental Objective (SDO): develop the capacities for ecophysiologically trained researchers to reduce climate change vulnerability of the coastal areas of Cuba.
Prof.Dr. Raúl Carlos López Sánchez
Local promoter

3. Prof.Dr. Raúl Carlos López Sánchez
Intermediate Results (IR)
IR1. The ecophysiological mechanisms for salt and drought stress tolerance of C. uvifera are known.
IR2. Nutritional and phytochemical compounds of C. uvifera are characterized and known.
IR3. The visibility of the Abiotic Stress Group of the Biotechnology Study Centre, as an excellence center for ecophysiological salt and drought stress research in Cuba has increased
Prof.Dr. Raúl Carlos López Sánchez
Local promoter

4. Prof.Dr. Raúl Carlos López Sánchez
IR1. The ecophysiological mechanisms for salt and drought stress tolerance of C. uvifera are known.
1.1. Preparation of experiments
1.1.2 Literature review about drought and salt stress in relation to the selected and similar species
Prof.Dr. Raúl Carlos López Sánchez
Local promoter

5. 1.1. Preparation of experiments
North
South
IR1. The ecophysiological mechanisms for salt and drought stress tolerance of C. uvifera are known.
1.1. Preparation of experiments
1.1.3 Definition of experimental design and practical requirements
Playa Guardalavaca, Holguin province
Latitude: 21°07′30″N
Longitude: 75°49′44″O
East
Playa Las Coloradas, Granma province
Latitude: 19° 56' 00" N
Longitude: 77° 41' 00" W
Playa Ancon, Sancti Spiritus province
Latitude: 21° 44' 00" N
Longitude: 80° 01' 00" W
Middle
Cayo Coco, Ciego de Avila province
Latitude: 22º 32' 10.06" N
Longitude: 78º 21' 19.44" W
West
Playa Varadero, Matanzas province
Latitude: 23°10'28"N
Longitude: 81°12'43"W
Playa Rancho Luna, Cienfuegos province
Latitude: 22°02′20″N
Longitude: 80°25′38″W

6. Prof.Dr. Raúl Carlos López Sánchez
IR1. The ecophysiological mechanisms for salt and drought stress tolerance of C. uvifera are known.
1.1. Preparation of experiments
1.1.3 Definition of experimental design and practical requirements
Prof.Dr. Raúl Carlos López Sánchez
Local promoter

7. 1.1. Preparation of experiments
IR1. The ecophysiological mechanisms for salt and drought stress tolerance of C. uvifera are known.
1.1. Preparation of experiments
1.1.5 Training of the personnel responsible for the manipulation of the equipment and the measurements

8. Prof.Dr. Raúl Carlos López Sánchez
IR1. The ecophysiological mechanisms for salt and drought stress tolerance of C. uvifera are known.
1.2. Experiments in controlled conditions
1.2.1 Evaluation of the response to salt and drought stress of C. uvifera during germination
Prof.Dr. Raúl Carlos López Sánchez
Local promoter

9. Prof.Dr. Raúl Carlos López Sánchez
Control
5 dS.m-1
IR1. The ecophysiological mechanisms for salt and drought stress tolerance of C. uvifera are known.
1.2. Experiments in controlled conditions
1.2.1 Evaluation of the response to salt and drought stress of C. uvifera during germination
15 dS.m-1
25 dS.m-1
Salt stress
Prof.Dr. Raúl Carlos López Sánchez
Local promoter

10. 50 % field capacity 100 % field capacity
IR1. The ecophysiological mechanisms for salt and drought stress tolerance of C. uvifera are known.
1.2. Experiments in controlled conditions
1.2.1 Evaluation of the response to salt and drought stress of C. uvifera during germination
Drought stress
0% field capacity

11. IR1. The ecophysiological mechanisms for salt and drought stress tolerance of C. uvifera are known.
1.2. Experiments in controlled conditions
1.2.2 Evaluation of ecophysiological responses (gas exchange, photosynthesis, chlorophyll fluorescence, plant-water-relations, leaf area index) of C. uvifera to salt and drought stress at seedling and sapling stage.
Control
15 dS.m-1
Salt stress

12. IR1. The ecophysiological mechanisms for salt and drought stress tolerance of C. uvifera are known.
1.2. Experiments in controlled conditions
1.2.2 Evaluation of ecophysiological responses (gas exchange, photosynthesis, chlorophyll fluorescence, plant-water-relations, leaf area index) of C. uvifera to salt and drought stress at seedling and sapling stage.
Control
15 dS.m-1
Salt stress
Control
15 dS.m-1

13. IR1. The ecophysiological mechanisms for salt and drought stress tolerance of C. uvifera are known.
1.2. Experiments in controlled conditions
1.2.2 Evaluation of ecophysiological responses (gas exchange, photosynthesis, chlorophyll fluorescence, plant-water-relations, leaf area index) of C. uvifera to salt and drought stress at seedling and sapling stage.
Salt stress

14. 1.3. Experiments in field conditions.
North
South
1.3. Experiments in field conditions.
1.3.2 Fruit collection of C. uvifera accessions in selected ecosystems.
Playa Guardalavaca, Holguin province
Latitude: 21°07′30″N
Longitude: 75°49′44″O
East
Playa Las Coloradas, Granma province
Latitude: 19° 56' 00" N
Longitude: 77° 41' 00" W

15. IR2. Nutritional and phytochemical compounds of C
IR2. Nutritional and phytochemical compounds of C. uvifera are characterized and known.
2.1 Intensive literature review on C. uvifera about the nutritional and medicinal compounds

16. 2.2 Evaluation of nutritional quality of C. uvifera
IR2. Nutritional and phytochemical compounds of C. uvifera are characterized and known.
2.2 Evaluation of nutritional quality of C. uvifera
2.3 Phytochemical screening and antibacterial activity evaluation of C. uvifera
Metabolites
North of east region
Leaves
Bark of the shaft
Fruits
DCM
EtOH
EtOAc
Alkaloids - + ++
Coumarins
Fatty Acid
Resins
Triterpenes/Steroids
Saponines* (ultrasonido) -*
Saponines* (maceración) +*
Free amino acid
Cardiotonic Glycosides
Reducers Carbohydrates
Phenols/Tanines
+++
Quinones
Antocianidines
Flavonoids
Better compounds
Mucilages

17. Prof.Dr. Raúl Carlos López Sánchez
Local promoter

18. Prof.Dr. Raúl Carlos López Sánchez
Local promoter

19. Prof.Dr. Raúl Carlos López Sánchez
Local promoter